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Appendix A: Cryotech® Vitrification Thawing.
Goral Gandhi1, Masashige Kuwayama2, Sakina Kagalwala1
1IVF Department, Rotunda-The Center For Human Reproduction, 36, Turner Road, B Wing, 101, Bandra (W), Mumbai, 400050, Maharashtra, India.
Methods in Molecular Biology (Clifton, N.J.)
|April 20, 2017
Summary
Vitrification is a preferred method for preserving human eggs and embryos, enhancing in vitro fertilization (IVF) success. The Cryotech method offers a minimal volume approach for improved survival and safety in cryopreservation.
Area of Science:
- Reproductive Medicine
- Cryobiology
Background:
- Vitrification has become a standard technique for human oocyte and embryo cryopreservation over the past 15-20 years.
- Advancements in vitrification technology have led to improved success rates and greater flexibility in assisted reproductive technologies.
Purpose of the Study:
- To describe the Cryotech method, a novel minimal volume approach for cryopreservation.
- To detail the application of this method for oocytes and embryos at all developmental stages, including blastocysts.
Main Methods:
- The Cryotech method, developed by Dr. Masashige Kuwayama, utilizes a minimal volume approach.
- The protocol focuses on accurate application for optimal survival and safety during cryopreservation.
Main Results:
- The Cryotech method is suitable for cryopreservation of human oocytes and embryos at any developmental stage.
- This technique aims for perfect survival and safety, contributing to more successful and flexible IVF treatments.
Conclusions:
- The Cryotech minimal volume approach represents a significant advancement in oocyte and embryo cryopreservation.
- This method enhances the success and flexibility of in vitro fertilization procedures.