Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Encapsulation into complex coacervate core micelles promotes EGFP dimerization.

A Nolles1, N J E van Dongen, A H Westphal

  • 1Laboratory of Biochemistry, Wageningen University & Research, Stippeneng 4, 6708 WE Wageningen, The Netherlands. janwillem.borst@wur.nl.

Physical Chemistry Chemical Physics : PCCP
|April 20, 2017
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Rheological characterisation of alginate-like exopolymer gels crosslinked with calcium.

Water research·2021
Same author

Self-consistent field modeling of mesomorphic phase changes of monoolein and phospholipids in response to additives.

Physical chemistry chemical physics : PCCP·2021
Same author

Electrode Surface Potential-Driven Protein Adsorption and Desorption through Modulation of Electrostatic, van der Waals, and Hydration Interactions.

Langmuir : the ACS journal of surfaces and colloids·2021
Same author

Structural and mechanical parameters of lipid bilayer membranes using a lattice refined self-consistent field theory.

Physical chemistry chemical physics : PCCP·2021
Same author

Step-wise linking of vesicles by combining reversible and irreversible linkers - towards total control on vesicle aggregate sizes.

Soft matter·2020
Same author

Self-limiting aggregation of phospholipid vesicles.

Soft matter·2020

Complex coacervate core micelles (C3Ms) encapsulate proteins, influencing their properties. Encapsulated enhanced green fluorescent protein (EGFP) dimerizes, unlike its monomeric form (mEGFP), altering spectroscopic features.

Area of Science:

  • Biophysics
  • Materials Science
  • Protein Chemistry

Background:

  • Complex coacervate core micelles (C3Ms) are effective colloidal structures for encapsulating biomacromolecules.
  • Previous work showed encapsulation of enhanced green fluorescent protein (EGFP) into C3Ms using a specific diblock copolymer alters its spectroscopic properties.

Purpose of the Study:

  • To investigate the impact of C3M encapsulation on monomeric EGFP (mEGFP) compared to EGFP.
  • To determine if EGFP dimerizes within C3Ms and understand the underlying mechanisms and consequences.

Main Methods:

  • Spectroscopic analysis (absorption, fluorescence, circular dichroism) of encapsulated EGFP and mEGFP.
  • Time-resolved fluorescence anisotropy measurements to study homo-Förster resonance energy transfer (FRET).

Related Experiment Videos

  • Investigating the reversibility of observed changes upon release from C3Ms.

    • Main Results:

    • Micellar encapsulation affects mEGFP's spectroscopic properties less than EGFP.
    • Circular dichroism changes are specific to encapsulated EGFP, suggesting structural alterations.
    • Homo-FRET analysis indicates EGFP dimerizes within C3Ms, while mEGFP remains monomeric.
    • Encapsulated EGFP dimerization leads to a reversible pKa shift of the chromophore.

    Conclusions:

    • EGFP dimerizes within C3Ms, a phenomenon not observed for mEGFP.
    • Dimerization-induced reorientation of Glu222 causes a chromophore pKa shift, reversible upon release.
    • C3M encapsulation provides insights into protein behavior in confined environments and potential applications in controlled release.