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miR-34b Modulates Skeletal Muscle Cell Proliferation and Differentiation
Zhixiong Tang1, Huiling Qiu1,2, Lan Luo1
1Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Sciences, Shenzhen University, Shenzhen, Guangdong, 518060, China.
Journal of Cellular Biochemistry
|April 20, 2017
Summary
MicroRNAs (miRNAs) regulate muscle development. miR-34b promotes myotube formation by repressing proliferation and targeting nucleolin, a protein essential for myogenesis.
Area of Science:
- Molecular Biology
- Cell Biology
- Developmental Biology
Background:
- Myogenesis, the process of muscle development, involves myoblast proliferation, differentiation, and fusion.
- MicroRNAs (miRNAs) are increasingly recognized as key regulators of myogenesis.
- miR-34b levels rise during myogenesis, but its specific role remains unclear.
Purpose of the Study:
- To investigate the function of miR-34b in myogenesis.
- To identify the targets of miR-34b during muscle cell differentiation.
- To elucidate the role of nucleolin in myogenesis.
Main Methods:
- Quantitative iTRAQ-based proteomic analysis in mouse myoblast C2C12 cells.
- Identification of miRNA targets through 3'UTR binding analysis.
- Manipulation of nucleolin levels to assess its impact on myogenesis.
Main Results:
- miR-34b was found to repress muscle cell proliferation and promote myotube formation.
- Proteomic analysis identified 97 proteins regulated by miR-34b, including 14-3-3 gamma, adenosylhomocysteinase, and nucleolin.
- Nucleolin was confirmed as a direct target of miR-34b, and its expression level influences myogenesis in a dose-dependent manner.
Conclusions:
- miR-34b plays a crucial role in regulating myogenesis by inhibiting proliferation and promoting differentiation.
- Nucleolin is a key target of miR-34b and its abundance critically controls myoblast proliferation and differentiation.
- These findings reveal a novel regulatory mechanism of myogenesis involving miR-34b and nucleolin.