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Related Experiment Videos

Poly(A) polymerase activity in murine serum. Elevation in animals with proliferative changes.

R N Kurl1, S C Holmes, E Verney

  • 1Department of Pathology, George Washington University Medical Center, Washington, DC 20037.

Journal of the National Cancer Institute
|September 7, 1988
PubMed
Summary

Normal rat sera contain poly(A) polymerase activity. This enzyme

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Enzymology

Background:

  • Polynucleotide adenylyltransferase, also known as poly(A) polymerase, plays a crucial role in RNA processing.
  • Investigating the presence and characteristics of poly(A) polymerase in biological fluids is essential for understanding cellular regulation.

Purpose of the Study:

  • To characterize the poly(A) polymerase activity found in normal rat sera.
  • To determine the conditions that optimize this enzymatic activity.
  • To investigate the presence of elevated poly(A) polymerase activity in specific disease states.

Main Methods:

  • Enzyme assays were performed to measure polynucleotide adenylyltransferase activity.
  • Optimization studies were conducted for primer concentration, ion requirements, kinetics, and protein levels.

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  • Inhibitors were used to confirm the enzymatic identity as poly(A) polymerase.
  • Enzymatic activity was quantified in sera from rats with transplanted hepatomas, ascites hepatoma, partial hepatectomy, and MRL/lpr mice with autoimmune conditions.

    • Main Results:

    • Normal rat sera exhibit measurable poly(A) polymerase activity.
    • The enzymatic activity was optimized, confirming its identity as poly(A) polymerase.
    • Significantly elevated levels of poly(A) polymerase activity were observed in sera from rats with hepatomas (transplanted and ascites), partially hepatectomized rats, and MRL/lpr mice with autoimmune disease.

    Conclusions:

    • Poly(A) polymerase is present and active in normal rat sera.
    • Elevated serum poly(A) polymerase activity is associated with specific conditions including hepatomas, liver regeneration, and autoimmune lymphoproliferation.
    • These findings suggest serum poly(A) polymerase may serve as a potential biomarker for certain pathological states.