Transcriptional co-activator YAP regulates cAMP signaling in Sertoli cells
Souvik Sen Sharma1, Subeer S Majumdar2
1Cellular Endocrinology Lab, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi, India.
Abstract:
FSH mediated cyclic AMP (cAMP) signaling is crucial for function of testicular Sertoli cells (Sc) during puberty. Yes-kinase Associated Protein (YAP), a transcriptional co-activator, regulates cell proliferation and differentiation. However, its role in testicular function is not known. In present study, we have identified YAP as an important regulator of cAMP signaling in Sc, in-vitro. Verteporfin, a YAP-inhibitor, down regulated the expression of cAMP responsive genes necessary for spermatogenesis in Sc. Action of forskolin, which acts via cAMP, was also antagonized by verteporfin, limiting expression of these genes. Assessment of cAMP-responsive-element-binding-protein (CREB) phosphorylation revealed that verteporfin augmented the phosphorylation of CREB at Ser133 residue. This effect of verteporfin on CREB phosphorylation was attenuated by H-89, the PKA inhibitor. This clearly suggested involvement of PKA in verteporfin mediated CREB phosphorylation. We provided evidence for the first time that YAP modulates cAMP signaling in Sc which may be critical for testicular function.
Related Concept Videos
Co-activators and Co-repressors
Co-activators and Co-repressors
RNA Polymerase II Accessory Proteins
Eukaryotic Transcription Activators
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These...
Master Transcription Regulators
Master Transcription Regulators


