Related Experiment Video
Updated: Mar 3, 2026
A Cost-Effective and Minimally Invasive Protocol for Chronic Multi-Site Electroencephalography Recording in Freely Moving Mice
Published on: October 10, 2025
A novel device for continuous long-term electroencephalogram recording and drug administration in mice with a nice,
Shigeru Watanabe1, Masanori Saito1, Masaki Soma2
1Department of Psychiatry, Kitasato University School of Medicine, 2-1-1 Asamizodai, Minami-ku, Sagamihara-shi, Kanagawa 252-0380, Japan.
Background:
To elucidate mechanisms of epileptogenesis and epileptic maturation, and to develop new AEDs, it is indispensable to administer various drugs and to examine their effects on EEG over a long period of observation.
New Method:
We constructed a device for the continuous measurement of electroencephalography (EEG) and the infusion of anti-epileptic drugs over a prolonged period of time in moving mice. The system includes a slip ring and a swivel to prevent twisting of the recording cable and infusion tube, respectively. We introduced three arms, ball bearing, and stabilizing frame to rotate the slip ring and swivel with only a small applied force, and to facilitate the start of rotation of the slip ring and the swivel.
Results:
Continuous EEG recording was successfully performed for up to 63 days in 99 mice, for a total of 1872 days of EEG data. Continuous drug infusion with continuous EEG recording was successfully performed for up to 22 days.
Comparison With Existing Method(S):
Our system is superior to current system in continuous drug delivery during long-term EEG recording in moving mouse.
Conclusions:
Our device will be quite useful for long-term EEG recording and drug application in moving mice.
