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Updated: Aug 12, 2026

TransFLP — A Method to Genetically Modify Vibrio cholerae Based on Natural Transformation and FLP-recombination
Published on: October 8, 2012
Controllable alteration of cell genotype in bacterial cultures using an excision vector
1BioTechnica International, Inc., Cambridge, MA 02140.
Abstract:
We have used recombinant DNA techniques to construct a derivative of phage lambda, called an excision vector, which retains only those functions necessary for conditional maintenance of lysogeny and integration/excision. The tyrA+ gene was cloned on this excision vector, integrated into the Escherichia coli chromosome, and stably maintained and expressed under permissive conditions. Upon shift to non-permissive conditions, the excision vector and its passenger gene were very efficiently excised from the chromosome and lost, leaving a culture of Tyr- bacteria. This illustrates a new class of conditional mutations in which the genotype changes in response to external stimuli.
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