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Multiphoton Intravital Imaging for Monitoring Leukocyte Recruitment during Arteriogenesis in a Murine Hindlimb Model

Multiphoton Intravital Imaging for Monitoring Leukocyte Recruitment during Arteriogenesis in a Murine Hindlimb Model

Published on: September 30, 2021

Multi-photon microscopy in cardiovascular research.

Zhuojun Wu¹, Timo Rademakers², Fabian Kiessling³

¹Institute for Molecular Cardiovascular Research, University Clinic, RWTH-Aachen University, Pauwelsstrasse 30, 52074 Aachen, Germany; Institute for Experimental Molecular Imaging, University Clinic, RWTH Aachen University, Pauwelsstrasse 30, 52074 Aachen, Germany.

Methods (San Diego, Calif.)

|April 25, 2017

Summary

Multiphoton microscopy enables detailed visualization of cardiovascular diseases in animal models. This advanced imaging technique offers potential for clinical translation in diagnosing and treating heart and vascular conditions.

Keywords:

Cardiovascular imaging Carotid artery Intravital imaging Non-linear microscopy Optical imaging

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Area of Science:

Cardiovascular research
Microscopy
Medical imaging

Background:

Multiphoton microscopy is valuable for ex vivo 3D histology and in vivo imaging.
Advances in triggering and fast imaging have enabled intravital imaging of dynamic cardiovascular structures.
Intravital multiphoton imaging is increasingly popular in cardiovascular research.

Purpose of the Study:

To review the potential of multiphoton microscopy for visualizing and characterizing cardiac and vascular diseases.
To highlight applications in preclinical cardiovascular research, including atherosclerosis and inflammation.
To discuss clinical translation possibilities for disease diagnosis and therapy.

Main Methods:

Review of multiphoton microscopy techniques.
Ex vivo 3D histology and in vivo imaging of animal models.
Intravital imaging of pulsating heart and major arteries.

Main Results:

Multiphoton microscopy allows subcellular visualization of atherosclerotic plaque development.
Intravital imaging can capture inflammatory processes in the heart.
The technique is effective for both ex vivo and intravital animal studies.

Conclusions:

Multiphoton microscopy holds significant potential for preclinical cardiovascular research.
The technology can be translated for clinical applications in cardiovascular disease diagnosis and treatment.
Advanced imaging provides new insights into dynamic and structural disease processes.