Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Feedback Regulation of Calcium Concentration01:27

Feedback Regulation of Calcium Concentration

4.1K
Calcium is an essential signaling molecule required for various cellular functions. Calcium pumps and ion channels on cell and organellar membranes, such as those on the endoplasmic reticulum (ER), regulate calcium concentrations inside the cell. They remain closed, keeping the cytosolic calcium levels low at a resting state.
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
4.1K
Calmodulin-dependent Signaling01:16

Calmodulin-dependent Signaling

6.8K
Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
6.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Inhibition of Wnt/β-catenin signal is alleviated reactive gliosis in rats with hydrocephalus.

Child's nervous system : ChNS : official journal of the International Society for Pediatric Neurosurgery·2015
Same author

Applications of data mining methods in the integrative medical studies of coronary heart disease: progress and prospect.

Evidence-based complementary and alternative medicine : eCAM·2014
Same author

Remediation of pharmaceuticals and personal care products using an aerobic granular sludge sequencing bioreactor and microbial community profiling using Solexa sequencing technology analysis.

Bioresource technology·2014
Same author

Anti-Inflammatory and Immunomodulatory Mechanism of Tanshinone IIA for Atherosclerosis.

Evidence-based complementary and alternative medicine : eCAM·2014
Same author

Anti-inflammatory immune skewing is atheroprotective: Apoe−/−FcγRIIb−/− mice develop fibrous carotid plaques.

Journal of the American Heart Association·2014
Same author

Standardization and future directions in pattern identification research: International brainstorming session.

Chinese journal of integrative medicine·2014

Related Experiment Video

Updated: Mar 3, 2026

Author Spotlight: Investigating Viral Disruption of Intestinal Epithelial Signaling – Research Insights and Future Directions
08:01

Author Spotlight: Investigating Viral Disruption of Intestinal Epithelial Signaling – Research Insights and Future Directions

Published on: January 19, 2024

2.0K

Quantum dots modulate intracellular Ca2+ level in lung epithelial cells.

Huijuan Yin1, Jacopo M Fontana1, Johan Solandt1,2

  • 1Section of Cellular Biophysics, Department of Applied Physics, Royal Institute of Technology (KTH), Science for Life Laboratory, Solna.

International Journal of Nanomedicine
|April 25, 2017
PubMed
Summary

Quantum dots (QDs) disrupt airway epithelial cells by increasing intracellular calcium, affecting electrical resistance. This nanoparticle interaction highlights potential pulmonary disease mechanisms.

Keywords:
Calu-3 epithelial layercell movementintracellular Ca2+ concentration [Ca2+]iquantum dottransepithelial electrical resistance

More Related Videos

Imaging Local Ca2+ Signals in Cultured Mammalian Cells
09:30

Imaging Local Ca2+ Signals in Cultured Mammalian Cells

Published on: March 3, 2015

11.5K
Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis
09:07

Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis

Published on: February 18, 2020

8.6K

Related Experiment Videos

Last Updated: Mar 3, 2026

Author Spotlight: Investigating Viral Disruption of Intestinal Epithelial Signaling – Research Insights and Future Directions
08:01

Author Spotlight: Investigating Viral Disruption of Intestinal Epithelial Signaling – Research Insights and Future Directions

Published on: January 19, 2024

2.0K
Imaging Local Ca2+ Signals in Cultured Mammalian Cells
09:30

Imaging Local Ca2+ Signals in Cultured Mammalian Cells

Published on: March 3, 2015

11.5K
Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis
09:07

Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis

Published on: February 18, 2020

8.6K

Area of Science:

  • Nanomedicine
  • Cell Biology
  • Pulmonary Science

Background:

  • Adverse effects of nanoparticles on lung health are known, but direct impacts on airway epithelium are understudied.
  • Understanding nanoparticle-epithelium interactions is crucial for assessing pulmonary risks.

Purpose of the Study:

  • To investigate the direct effects of quantum dots (QDs) on the Calu-3 airway epithelial cell layer.
  • To measure intracellular calcium ([Ca2+]i) changes and transepithelial electrical resistance (TEER) in response to QD exposure.

Main Methods:

  • Calu-3 cells were exposed to 3-mercaptopropionic-acid (3MPA) coated CdSe-CdS/ZnS core-multishell QDs.
  • Intracellular Ca2+ concentration ([Ca2+]i) and TEER were measured simultaneously.
  • Pharmacological inhibitors (GdCl3, BTP2, nifedipine) and Ca2+-free medium were used to probe calcium channel involvement.

Main Results:

  • QD deposition caused a transient decrease in TEER and a global increase in [Ca2+]i, with cell shape changes.
  • The [Ca2+]i increase was rapid apically and decreased basolaterally.
  • QD-induced TEER changes and Ca2+ entry were inhibited by SA and SOCE inhibitors and Ca2+-free conditions.
  • Nifedipine partially inhibited TEER response but not [Ca2+]i increase, indicating Ca2+ influx via SA and SOCE channels.

Conclusions:

  • Quantum dots induce Ca2+ influx in airway epithelium through mechanical stretch-activated (SA) and store-operated calcium entry (SOCE) channels.
  • The timing of [Ca2+]i increase and TEER drop suggests distinct cellular responses to QD exposure.
  • This study offers novel insights into direct nanoparticle-airway epithelium interactions and potential pulmonary disease mechanisms.