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Genetic translocation in Staphylococcus aureus.

R P Novick, I Edelman, M D Schwesinger

    Proceedings of the National Academy of Sciences of the United States of America
    |January 1, 1979
    PubMed
    Summary
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    The transposable element Tn551 in Staphylococcus aureus can move independently of host DNA repair mechanisms. This transposon also typically excises precisely, a rare trait among mobile genetic elements.

    Area of Science:

    • Bacteriology
    • Molecular Biology
    • Genetics

    Background:

    • Staphylococcus aureus is a significant Gram-positive bacterial pathogen.
    • Transposable elements (TEs) are mobile genetic sequences that can alter host genomes.
    • Understanding TE behavior is crucial for microbial genetics and evolution.

    Purpose of the Study:

    • To characterize the transposition and excision properties of the novel transposon Tn551 in Staphylococcus aureus.
    • To investigate the mechanism of Tn551 transposition, particularly its dependence on host recombination pathways.
    • To explore the potential of UV-induced plasmid inactivation as a method for detecting transposable elements.

    Main Methods:

    • Isolation and characterization of the 5.2-kilobase pair transposon Tn551 from Staphylococcus aureus plasmid pI258.

    Related Experiment Videos

  • Analysis of Tn551 transposition to various chromosomal and plasmid sites.
  • Assessment of the rec-independence of Tn551 transposition.
  • Investigation of Tn551 excision events.
  • Utilizing UV inactivation of the carrier plasmid to observe transposition.

    • Main Results:

    • Tn551 was identified in Staphylococcus aureus, residing initially on plasmid pI258.
    • Tn551 exhibited rec-independent transposition, moving to multiple genomic locations.
    • Transposition events occasionally resulted in insertional inactivation of target genes.
    • Tn551 demonstrated a characteristic feature of precise excision, distinguishing it from many other transposons.
    • UV inactivation of the host plasmid was effective in inducing and observing Tn551 transposition.

    Conclusions:

    • Tn551 is a unique transposable element in Staphylococcus aureus with rec-independent transposition and precise excision capabilities.
    • The rec-independent nature of Tn551 transposition suggests novel mechanisms.
    • Precise excision is a key characteristic of Tn551, offering potential advantages in genetic manipulation.
    • UV-induced plasmid inactivation serves as a viable method for detecting and studying transposable elements like Tn551.