Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Click Capture SELEX for the Identification of Click-Modified Aptamers Targeting Small Molecules in Solution.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same author

Selection and Characterization of SARS-CoV-2 Spike Binding Clickmers.

Chembiochem : a European journal of chemical biology·2026
Same author

Lighting the spark of aptamer data science.

Science (New York, N.Y.)·2026
Same author

Exploring an Aptamer-Based Approach to Assess Canine Parvovirus Integrity After Disinfection Treatment.

Viruses·2025
Same author

Robotic-Assisted Capture-Systematic Evolution of Ligands by Exponential Enrichment of RNA Aptamers Binding to Small Molecules.

Chembiochem : a European journal of chemical biology·2025
Same author

Optoribogenetic Modulation of Transcription.

Methods in molecular biology (Clifton, N.J.)·2024

Related Experiment Video

Updated: Mar 3, 2026

Primer-Free Aptamer Selection Using A Random DNA Library
11:14

Primer-Free Aptamer Selection Using A Random DNA Library

Published on: July 26, 2010

25.5K

Customised nucleic acid libraries for enhanced aptamer selection and performance.

Franziska Pfeiffer1, Malte Rosenthal1, Julia Siegl1

  • 1Life and Medical Sciences Institute, University of Bonn, Gerhard-Domagk-Str. 1, 53121 Bonn, Germany.

Current Opinion in Biotechnology
|April 25, 2017
PubMed
Summary

Modified aptamers with expanded chemical diversity overcome limitations of natural nucleobases. These advanced aptamers, including SOMAmers, enable targeting previously inaccessible molecules for biotechnology and therapeutics.

More Related Videos

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes
06:52

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

Published on: November 1, 2019

8.8K
Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
09:33

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Published on: March 21, 2018

10.4K

Related Experiment Videos

Last Updated: Mar 3, 2026

Primer-Free Aptamer Selection Using A Random DNA Library
11:14

Primer-Free Aptamer Selection Using A Random DNA Library

Published on: July 26, 2010

25.5K
Kinetic Screening of Nuclease Activity using Nucleic Acid Probes
06:52

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

Published on: November 1, 2019

8.8K
Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
09:33

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Published on: March 21, 2018

10.4K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biotechnology

Background:

  • Aptamers are short oligonucleotides with high affinity and specificity for target molecules.
  • Their application as molecular probes in biotechnology and therapeutics is promising.
  • Natural nucleobases limit aptamer interactions with diverse targets.

Purpose of the Study:

  • To explore methods for broadening the chemical diversity of aptamers.
  • To address the limitations imposed by natural nucleobases in aptamer development.
  • To review modified aptamers and their characteristics.

Main Methods:

  • Nucleobase modifications to create novel aptamers.
  • Introduction of non-natural bases and base pairs.
  • Development of aptamers with expanded genetic alphabets.

Main Results:

  • Modified aptamers exhibit enhanced targeting capabilities.
  • SOMAmers (slow off-rate modified aptamers) demonstrate improved binding characteristics.
  • Clickmers and aptamers with expanded genetic alphabets show versatile applicability.

Conclusions:

  • Broadening aptamer chemical diversity is crucial for expanding their utility.
  • Modified aptamers offer solutions for targeting previously inaccessible molecules.
  • These advancements position aptamers as versatile tools in molecular probing and therapeutics.