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Performance of MALDI-TOF MS for identification of oral Prevotella species

Mervi Gürsoy1, Inka Harju2, Jaakko Matomäki3

  • 1Department of Periodontology, Institute of Dentistry, University of Turku, Lemminkäisenkatu 2, FI-20520 Turku, Finland.

Anaerobe
|April 26, 2017
PubMed

Insights

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) accurately identifies Prevotella bacteria, crucial for diagnosing oral and non-oral infections. This rapid method surpasses traditional biochemical testing for reliable bacterial identification.

Area of Science:

  • Medical Microbiology
  • Bacterial Taxonomy
  • Diagnostic Technology

Background:

  • The genus Prevotella is clinically significant, implicated in diverse oral and non-oral infections.
  • Accurate identification of Prevotella species is essential for effective clinical management.
  • Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a modern technique for bacterial identification.

Purpose of the Study:

  • To evaluate the diagnostic accuracy of MALDI-TOF MS for identifying Prevotella species from clinical oral isolates.
  • To compare the performance of MALDI-TOF MS against 16S rRNA sequencing as a reference standard.
  • To assess the reliability of MALDI-TOF MS for Prevotella identification in clinical microbiology.

Main Methods:

  • 123 oral Prevotella isolates were analyzed using Bruker MALDI-TOF MS.
  • Isolates were selected based on biochemical profiling.
  • Partial 16S rRNA gene sequencing served as the reference method for identification.

Main Results:

  • MALDI-TOF MS achieved 100.0% accuracy for genus-level identification of Prevotella.
  • Species-level identification accuracy was 88.6% with a log score of ≥2.0.
  • Prevotella aurantiaca and P. jejuni, not in the database, were correctly identified to the genus level.

Conclusions:

  • MALDI-TOF MS is a reliable and rapid method for identifying Prevotella species within its database.
  • The technique demonstrates high accuracy for genus-level identification and good performance at the species level.
  • MALDI-TOF MS offers a significant advantage over biochemical testing for Prevotella identification.
Keywords:
Anaerobic bacteriaMALDI-TOF MSMolecular diagnostic techniquesMouth

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