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DNAase I footprinting of restriction enzymes
1Dept. of Physiology and Pharmacology University of Southampton, U.K.
Biochemical and Biophysical Research Communications
|September 15, 1988
Abstract:
DNAase I footprinting of restriction enzymes has been achieved by using calcium containing digestion buffers so that the enzymes bind to but do not cleave DNA. EcoR1 produces a footprint 17 bases long, overestimating the region of contact with DNA by about 7-8 base pairs. Restriction enzymes HaeIII and HinP1 generate smaller footprints of 15 and 13 base pairs respectively.