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Updated: Mar 3, 2026

Studying Age-dependent Genomic Instability using the S. cerevisiae Chronological Lifespan Model
Published on: September 29, 2011
Generation of mutation hotspots in ageing bacterial colonies
Agnieszka Sekowska1, Sofie Wendel2, Emil C Fischer2
1Institute of Cardiometabolism and Nutrition, CHU Pitié-Salpêtrière, 47, Boulevard de l'Hôpital, 75013, Paris, France.
Abstract:
How do ageing bacterial colonies generate adaptive mutants? Over a period of two months, we isolated on ageing colonies outgrowing mutants able to use a new carbon source, and sequenced their genomes. This allowed us to uncover exquisite details on the molecular mechanism behind their adaptation: most mutations were located in just a few hotspots in the genome, and over time, mutations increasingly were consistent with the involvement of 8-oxo-guanosine, formed exclusively on the transcribed strand. This work provides strong support for retromutagenesis as a general process creating adaptive mutations during ageing.
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