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Updated: Aug 22, 2026

Removal of Exogenous Materials from the Outer Portion of Frozen Cores to Investigate the Ancient Biological Communities Harbored Inside
Published on: July 3, 2016
Psychrophilic Bacteria and Enzyme Activity in Antarctic Ice Environments
Dilara Devecioglu1, Dilara Nur Dikmetas1, Gamze Neşe-Özcan2
1Istanbul Technical University, Faculty of Chemical and Metallurgical Engineering, Department of Food Engineering, Maslak, Istanbul 34449, Türki̇ye.
None:
Psychrophilic organisms exhibit many adaptation mechanisms that enable them to survive at low temperatures ranging from -20 to 20 °C. Among other characteristics, these organisms produce cold-active enzymes with considerable biotechnological potential. The purpose of this study was to isolate and characterize bacteria from ice core samples collected on Horseshoe, Hovgaard, and Nansen Islands. Microorganisms from the latter two locations have been investigated only to a limited extent to date. Based on the phylogenetic analysis of 16S rRNA nucleotide sequences, eight bacterial isolates, including Sporosarcina, Arthrobacter, Bacillus, Luteimonas, Rhodococcus, and Methylobacterium were ultimately isolated and identified. Additionally, Raman and Fourier Transform Infrared Spectroscopy were used to analyze the biochemical properties of the bacteria. The bacterial culture's enzyme activity was assessed utilizing semiquantitative and screening techniques, and the API ZYM analysis revealed high enzyme activity, particularly for naphthol-AS-BI-phosphohydrolase, leucine aminopeptidase, esterase, lipase, and esterase. This study clarifies the biochemical characteristics of Antarctic bacteria and offers new perspectives on their enzymatic activity at various temperatures and resistance to antibiotics.
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