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Updated: Aug 15, 2026

Analytical Techniques for Assaying Nitric Oxide Bioactivity
Published on: June 18, 2012
Nitric oxide complex of cytochrome c' in cells of denitrifying bacteria
T Yoshimura1, H Iwasaki, S Shidara
1Environmental Science Insitute of Hyogo Prefecture, Kobe.
Abstract:
Electron paramagnetic resonance (EPR) spectra at 77K have been measured for the dried cells of three groups of chemoheterotrophic bacteria cultured in the presence of nitrate: I, denitrifying bacteria containing cytochrome c' (Alcaligenes sp. NCIB 11015, Achromobacter xylosoxidans GIFU 543); II, denitrifying bacteria in which cytochrome c' has not been found (Alcaligenes faecalis IAM 1015, Bacillus firmus NIAS 237, Pseudomonas stutzeri ATCC 17641); III, non-denitrifying bacterium containing cytochrome c' (A. xylosoxidans GIFU 1055). A characteristic three-line EPR signal was detected only in the dried cells of group I, and was assigned to NO-cytochrome c' complex. The probable physiological role of the cytochrome c' in denitrifying bacteria is discussed.
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