Related Experiment Video
Updated: Mar 3, 2026
08:33
Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants
Published on: August 5, 2020
8.8K
Construction of a map-based reference genome sequence for barley, Hordeum vulgare L
Sebastian Beier1, Axel Himmelbach1, Christian Colmsee1
1Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, 06466 Seeland, Germany.
Scientific Data
|April 28, 2017
Summary
The International Barley Genome Sequencing Consortium sequenced and assembled over 80,000 bacterial artificial chromosome (BAC) clones. This created a high-quality reference genome sequence for barley (Hordeum vulgare L.), improving genomic resources.
Area of Science:
- Plant genomics
- Agricultural science
- Bioinformatics
Background:
- Barley (Hordeum vulgare L.) is a vital cereal crop used for animal feed and malting.
- A high-quality reference genome sequence is crucial for advancing barley research and breeding.
- Previous genomic efforts have laid the groundwork for a comprehensive barley genome assembly.
Purpose of the Study:
- To report the detailed experimental and computational procedures for constructing the map-based reference genome sequence of barley cv. 'Morex'.
- To integrate diverse genomic data for a robust and ordered barley genome assembly.
- To provide an accessible platform for exploring the barley genome sequence and mapping resources.
Main Methods:
- Hierarchical shotgun sequencing of over 80,000 bacterial artificial chromosome (BAC) clones.
- Identification and validation of overlaps between adjacent BAC clones to build sequence scaffolds.
- Integration of genetic maps, optical maps, and chromosome conformation capture sequencing (Hi-C) data for chromosome-level ordering and orientation.
- Development of the Barley Genome Explorer (BARLEX) for data access.
Main Results:
- Assembly of more than 80,000 BAC clones into 4,265 non-redundant sequence scaffolds.
- Successful ordering and orientation of BAC clusters along the seven barley chromosomes.
- Creation of a high-quality, map-based reference genome sequence for barley cv. 'Morex'.
- Establishment of the BARLEX resource for integrative access to genomic data.
Conclusions:
- The study successfully generated a comprehensive, chromosome-level reference genome sequence for barley.
- The developed genomic resources and the BARLEX platform significantly enhance barley research capabilities.
- This achievement provides a foundation for future genetic and breeding advancements in barley.
Related Concept Videos
03:36Genome Annotation and Assembly
21.2K
The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
21.2K
02:54Evolutionary Relationships through Genome Comparisons
7.1K
Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
7.1K