Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

RNA Stability

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...

mRNA Stability and Gene Expression

mRNA Stability and Gene Expression

The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability

mRNA Stability and Gene Expression

mRNA Stability and Gene Expression

Nuclear Export of mRNA

Nuclear Export of mRNA

Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...

Nuclear Export of mRNA

Nuclear Export of mRNA

Regulation of Expression at Multiple Steps

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Publisher Correction: Interplay between cohesin and RNA polymerase II in regulating chromatin interactions and gene transcription.

Nature structural & molecular biology·2026

Same author

RepliSage: a stochastic graph-based framework for 3D chromatin modeling across the cell cycle.

Nucleic acids research·2026

Same author

HDA6 represses the histone acetylation modification and expression of WRKY18/40 to inhibit camalexin biosynthesis in Arabidopsis.

Plant cell reports·2026

Same author

Cohesin extrudes chromatin loop unidirectionally through two modes of mechanisms in human cells.

bioRxiv : the preprint server for biology·2026

Same author

Simultaneous modeling of chromatin conformation changes from multiple single-cell interaction maps with ChromMovie.

Genome research·2026

Same author

Topologically associating domains define the 3D genome architecture of mouse totipotent-like stem cells.

The Journal of biological chemistry·2026

Search research articles

Related Experiment Video

Updated: Mar 3, 2026

Assessment of Selective mRNA Translation in Mammalian Cells by Polysome Profiling

Assessment of Selective mRNA Translation in Mammalian Cells by Polysome Profiling

Published on: October 28, 2014

Cellular polyamines modulate mRNA stability.

Haoxi Chai¹, Wannian Yang², Huazhong Shi^1,2

¹a Department of Chemistry and Biochemistry , Texas Tech University , Lubbock , TX , USA.

Plant Signaling & Behavior

|April 28, 2017

Summary

This summary is machine-generated.

Polyamines (PAs) are vital molecules that help plants survive heat stress. The LHR1/PUT3 transporter stabilizes heat-responsive gene mRNAs, crucial for plant adaptation to high temperatures.

Keywords:

Gene regulation LHR1 PUT3 heat stress mRNA stability polyamine transporter

More Related Videos

Efficient Transcriptionally Controlled Plasmid Expression System for Investigation of the Stability of mRNA Transcripts in Primary Alveolar Epithelial Cells

Efficient Transcriptionally Controlled Plasmid Expression System for Investigation of the Stability of mRNA Transcripts in Primary Alveolar Epithelial Cells

Published on: March 6, 2020

Investigation of RNA Synthesis Using 5-Bromouridine Labelling and Immunoprecipitation

Investigation of RNA Synthesis Using 5-Bromouridine Labelling and Immunoprecipitation

Published on: May 3, 2018

Related Experiment Videos

Last Updated: Mar 3, 2026

Assessment of Selective mRNA Translation in Mammalian Cells by Polysome Profiling

Assessment of Selective mRNA Translation in Mammalian Cells by Polysome Profiling

Published on: October 28, 2014

Efficient Transcriptionally Controlled Plasmid Expression System for Investigation of the Stability of mRNA Transcripts in Primary Alveolar Epithelial Cells

Efficient Transcriptionally Controlled Plasmid Expression System for Investigation of the Stability of mRNA Transcripts in Primary Alveolar Epithelial Cells

Published on: March 6, 2020

Investigation of RNA Synthesis Using 5-Bromouridine Labelling and Immunoprecipitation

Investigation of RNA Synthesis Using 5-Bromouridine Labelling and Immunoprecipitation

Published on: May 3, 2018

Area of Science:

Plant Biology
Molecular Biology
Genetics

Background:

Polyamines (PAs) are essential polycationic compounds regulating growth and survival in all organisms.
PAs interact with anionic macromolecules like DNA, RNA, and proteins, modulating their functions.
LHR1/PUT3 is a plasma membrane-localized polyamine influx transporter in Arabidopsis.

Purpose of the Study:

To investigate the role of LHR1/PUT3 in stabilizing heat stress-responsive gene mRNAs.
To discuss a potential pathway for modulating PUT3 transport activity.
To explore the significance of evolutionary variations in PUT3.

Main Methods:

Analysis of LHR1/PUT3 function in Arabidopsis under heat stress conditions.
Investigating mRNA stabilization of heat-responsive genes.
Bioinformatic analysis of evolutionary variations in PUT3.

Main Results:

LHR1/PUT3 plays a critical role in stabilizing mRNAs of key heat stress responsive genes at high temperatures.
Evidence for a putative pathway regulating PUT3 transport activity was discussed.
Evolutionary variations in PUT3 were highlighted for their significance.

Conclusions:

LHR1/PUT3 is essential for plant thermotolerance by maintaining the stability of critical heat-response mRNAs.
Understanding PUT3 regulation and evolution can provide insights into improving plant stress resilience.
This work contributes to the knowledge of polyamine transport and its role in plant stress responses.