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Vitamin C and Vitamin E Protected B95-8 and Balb/c-3T3 Cells from Apoptosis Induced by Intermittent 50Hz ELF-EMF
Zhen Ding1, Jintao Li1,2, Fan Li1,2
1Beijing Key Laboratory of Environmental and Viral Oncology, School of Life Science and Bio-Engineering, Beijing University of Technology, Beijing, 100124, China.
Background:
The extremely low-frequency electromagnetic field (ELF-EMF), mainly emitted by electric transmission lines and household electronic appliances, is becoming a worldwide health risk. It is imperative to investigate the biological impacts of ELF-EMF and to identify products that are resistant to the radiation from 50 Hz ELF-EMF. In this study, we investigated the biological impacts of apoptosis caused by 50 Hz Power line ELF-EMF and the protective effects of Vit C and Vit E.
Methods:
We conducted this study in Beijing, China in 2013. B95-8 and Balb/c-3T3 cells were divided into a sham group, an expo group and 3 expo groups in which the cells were preincubated with various concentrations of Vit C and Vit E. Then, all of the cells were exposed to 50 Hz Power line ELF-EMF and examined for apoptosis. The cells were collected for apoptosis detection after exposure.
Results:
The percent of cells that undergoing apoptosis and preincubated with various concentrations of Vit C and Vit E were significantly lower than in the Expo group.
Conclusion:
Vit C and Vit E exert significant protective effects from 50 Hz ELF-EMF radiation. The optimal protective concentrations of Vit C and Vit E are 10 μmol/L and 25 μmol/L, respectively. The protective effect of vitamins was more apparent for Balb/c-3T3 cells than B95-8 cells.
