Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Reporter Genes02:11

Reporter Genes

13.5K
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
13.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

SAM-dependent methyltransferases: from chemoenzymatic natural product synthesis to industrial application.

Natural product reports·2026
Same author

A Ferrocene Metal-Ligand Triplet Diradical with a Terminal Iminyl Group Discovered by Time-Resolved Mid-Infrared Spectroscopy.

Journal of the American Chemical Society·2026
Same author

Decomposition of Magnetic Coupling in μ-Oxo-Bridged Metal Complexes.

Journal of chemical theory and computation·2026
Same author

Accurate <sup>19</sup>F NMR chemical shifts in Fe(II) complexes with the LH20t local hybrid functional.

Physical chemistry chemical physics : PCCP·2026
Same author

Stereoselective Prenylation of Aryl- and Heteroaryl Halides: γ-Selective Suzuki-Miyaura Coupling as a Tool for Asymmetric Csp<sup>2</sup>-Csp<sup>3</sup> Cross-Coupling.

The Journal of organic chemistry·2026
Same author

Evaluating Spin-Orbit Effects on the Thermochemistry of Proton-Coupled Electron Transfer.

Inorganic chemistry·2025

Related Experiment Video

Updated: Mar 3, 2026

Light-Controlled Fermentations for Microbial Chemical and Protein Production
08:37

Light-Controlled Fermentations for Microbial Chemical and Protein Production

Published on: March 22, 2022

4.8K

Light-controlled gene expression in yeast using photocaged Cu2.

Peter M Kusen1, Georg Wandrey2, Vera Krewald3

  • 1Institute for Bioorganic Chemistry, Heinrich Heine University Düsseldorf at the Forschungszentrum Jülich, 52426 Jülich, Germany.

Journal of Biotechnology
|April 30, 2017
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel caged copper (Cu2+) system for precise, light-activated control of gene expression. This breakthrough enables automated, temporal, and quantitative regulation of recombinant protein production, offering a minimally invasive alternative to traditional methods.

Keywords:
BioLectorCaged compoundDM-nitrophen (PubChem CID: 3084)DMNP-EDTALight-regulated expressionOptogeneticspCUP1

More Related Videos

Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters
09:22

Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters

Published on: November 26, 2013

15.1K
Design and Implementation of an Automated Illuminating, Culturing, and Sampling System for Microbial Optogenetic Applications
11:13

Design and Implementation of an Automated Illuminating, Culturing, and Sampling System for Microbial Optogenetic Applications

Published on: February 19, 2017

10.2K

Related Experiment Videos

Last Updated: Mar 3, 2026

Light-Controlled Fermentations for Microbial Chemical and Protein Production
08:37

Light-Controlled Fermentations for Microbial Chemical and Protein Production

Published on: March 22, 2022

4.8K
Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters
09:22

Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters

Published on: November 26, 2013

15.1K
Design and Implementation of an Automated Illuminating, Culturing, and Sampling System for Microbial Optogenetic Applications
11:13

Design and Implementation of an Automated Illuminating, Culturing, and Sampling System for Microbial Optogenetic Applications

Published on: February 19, 2017

10.2K

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Chemical Biology

Background:

  • Cellular function manipulation, including gene expression, is crucial for biotechnology.
  • Photolabile protecting groups enable light-activated control of effector molecules.
  • Existing caged compound systems for gene expression are limited.

Purpose of the Study:

  • To develop a novel caged metal ion system for light-mediated control of gene expression.
  • To utilize the photolabile chelator DMNP-EDTA for controlled Cu2+ release.
  • To establish the first caged metal ion-based system for regulating recombinant gene expression.

Main Methods:

  • Computational chemistry guided the use of DMNP-EDTA for Cu2+ release.
  • UV-A irradiation was used to activate the caged Cu2+ system.
  • A high-throughput screening system with LED arrays monitored eYFP expression for optimization.

Main Results:

  • Successfully demonstrated light-mediated control of the Cu2+-inducible pCUP1 promoter using caged Cu2+.
  • Optimized induction time and expression levels for eYFP.
  • Achieved parallel, automated, and optical regulation of gene expression.

Conclusions:

  • The DMNP-EDTA-Cu system represents the first caged metal ion for recombinant gene expression control.
  • This method offers precise temporal and quantitative regulation, superior to conventional induction.
  • Enables minimally invasive, automated, and parallel optical control of gene expression.