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Dual-color Correlative Light and Electron Microscopy for the Visualization of Interactions between Mitochondria and Lysosomes
Published on: September 27, 2024
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Analyzing Lysosome-Related Organelles by Electron Microscopy
Ilse Hurbain1,2,3, Maryse Romao1,2,3, Ptissam Bergam1,2,3,4
1Institut Curie, PSL Research University, CNRS, UMR 144, F-75005, Paris, France.
Methods in Molecular Biology (Clifton, N.J.)
|May 1, 2017
Summary
High-resolution electron microscopy is crucial for studying lysosome-related organelles (LROs). This study details transmission electron microscopy protocols for visualizing LRO morphology and molecular composition.
Area of Science:
- Cell Biology
- Microscopy Techniques
- Organelle Biology
Background:
- Intracellular organelles possess unique morphologies discernible via electron microscopy.
- Optical imaging offers insights into organelle localization and dynamics.
- Understanding lysosome-related organelles (LROs) requires high-resolution visualization.
Purpose of the Study:
- To provide detailed protocols for studying LROs using transmission electron microscopy (TEM).
- To highlight the utility of TEM for characterizing LRO morphology and molecular composition.
Main Methods:
- Transmission electron microscopy (TEM) for ultrastructural analysis.
- Conventional electron microscopy and its advancements.
- Immunoelectron microscopy for component localization and quantitative analysis.
Main Results:
- Detailed protocols for TEM-based LRO study are presented.
- TEM enables comprehensive investigation of LRO morphology.
- Immunoelectron microscopy allows for precise localization and quantification of LRO components.
Conclusions:
- Electron microscopy is essential for detailed LRO characterization.
- The provided protocols facilitate high-resolution studies of LROs.
- TEM and immunoelectron microscopy are powerful tools for deciphering LRO biogenesis, function, and dysfunction.
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