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Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry
Published on: March 17, 2015
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Detection of Lysosomal Exocytosis in Platelets by Flow Cytometry
Anna L Södergren1, Sofia Ramström2,3
1Clinical Chemistry, Department of Clinical and Experimental Medicine, Linköping University, SE-58185, Linköping, Sweden. anna.sodergren@liu.se.
Methods in Molecular Biology (Clifton, N.J.)
|May 1, 2017
Abstract:
Flow cytometry is a method that allows high throughput analysis of individual cells in suspension. By inclusion of fluorescently labelled antibodies, it is possible to analyze the abundance of one or more surface antigens, such as LAMP-1, without prior lysis of cells. Here we describe the special considerations required for the investigation of lysosomal exocytosis from platelets analyzed with flow cytometry.
