Indigo Carmine-Cu complex probe exhibiting dual colorimetric/fluorimetric sensing for selective determination of mono...

A new selective probe based on copper complex of Indigo Carmine (IC-Cu₂) for colorimetric, naked-eye, and fluorimetric recognition of mono hydrogen phosphate (MHP) ion in H₂O/DMSO (4:1v/v, 1.0mmolL^-1 HEPES buffer solution pH7.5) was developed. Detection limit of HPO₄^2- determination, achieved by fluorimetric and ₃lorimetric method, are 0.071 and 1.46μmolL^-1, respectively. Potential, therefore is clearly available in IC-Cu₂ complex to detect HPO₄^2- in micromolar range via dual visible color change and fluorescence response. Present method shows high selectivity toward HPO₄^2- over other phosphate species and other anions and was successfully utilized for analysis of P₂O₅ content of a fertilizer sample. The results obtained by proposed chemosensor presented good agreement with those obtained the colorimetric reference method. INHIBIT and IMPLICATION logic gates operating at molecular level have been achieved using Cu²⁺and HPO₄^2- as chemical inputs and UV-Vis absorbance signal as output.