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Related Experiment Video

Updated: Mar 3, 2026

Initial Evaluation of Antibody-conjugates Modified with Viral-derived Peptides for Increasing Cellular Accumulation and Improving Tumor Targeting
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Development of ADCs Using Molecular Imaging.

Masahiro Yasunaga1, Shino Manabe2, Atsushi Tsuji3

  • 1Division of Developmental Therapeutics, EPOC, National Cancer Center.

Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan
|May 2, 2017
PubMed
Summary
This summary is machine-generated.

Molecular imaging techniques effectively assess antibody delivery and controlled drug release for antibody-drug conjugates (ADCs). This approach aids in developing more effective ADCs for cancer therapy by evaluating drug accumulation and reducing toxicity.

Keywords:
MS imagingantibodyantibody-drug conjugatedrug delivery systemmolecular imaging

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Area of Science:

  • Oncology
  • Biotechnology
  • Molecular Imaging

Background:

  • Antibody-drug conjugates (ADCs) require specific antibodies and linkers for efficient drug delivery.
  • Controlled drug release (CDR) is crucial for ADC efficacy and safety.
  • Molecular imaging offers advanced tools to evaluate ADC performance.

Purpose of the Study:

  • To evaluate antibody delivery and CDR using molecular imaging techniques.
  • To demonstrate the utility of in vivo fluorescence imaging and PET imaging for ADC assessment.
  • To showcase a novel drug imaging system for CDR evaluation.

Main Methods:

  • In vivo fluorescence imaging for antibody penetration in lymphoma and pancreatic tumors.
  • Positron emission tomography (PET) with 89Zr-labeled antibody for tumor targeting in a spontaneous carcinogenesis model.
  • Microscopic mass spectroscopy (MMS) for evaluating CDR of paclitaxel (PTX)-loaded micelles and ADCs.

Main Results:

  • In vivo fluorescence and PET imaging confirmed antibody delivery and tumor targeting.
  • Micelles demonstrated selective tumor accumulation and reduced normal tissue toxicity via the EPR effect.
  • The MMS system successfully evaluated CDR for both micelle carriers and ADCs.

Conclusions:

  • Molecular imaging is a powerful strategy for developing and optimizing antibody-drug conjugates.
  • The developed drug imaging system enables precise evaluation of controlled drug release in ADCs.
  • This research contributes to the advancement of targeted cancer therapies using ADCs.