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Related Experiment Video

Updated: Mar 3, 2026

Tibial Nerve Transection - A Standardized Model for Denervation-induced Skeletal Muscle Atrophy in Mice
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Denervation does not Induce Muscle Atrophy Through Oxidative Stress.

Eva Pigna1, Emanuela Greco1, Giulio Morozzi2

  • 1DAHFMO Unit of Histology and Medical Embryology, InterUniversity Institute of Myology, Sapienza University of Rome, Rome, Italy.

European Journal of Translational Myology
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PubMed
Summary

Denervation causes muscle atrophy via catabolic pathways and oxidative stress. Antioxidant treatments did not prevent muscle mass loss, suggesting oxidative stress isn't the sole cause of neurogenic muscle atrophy.

Keywords:
antioxidant drugdenervationneurogenic muscle atrophyoxidative stress

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Area of Science:

  • Muscle physiology and molecular biology
  • Neurodegenerative diseases
  • Skeletal muscle atrophy research

Background:

  • Denervation activates catabolic pathways (ubiquitin-proteasome, autophagy), leading to skeletal muscle atrophy and weakness.
  • Oxidative stress is induced by denervation and implicated in skeletal muscle atrophy.
  • Molecular pathways of muscle atrophy in denervation-related diseases are not fully understood.

Purpose of the Study:

  • To investigate the kinetics of oxidative stress response activation in skeletal muscle after denervation.
  • To determine the role of oxidative stress in neurogenic muscle atrophy.

Main Methods:

  • Analysis of oxidative stress response kinetics in skeletal muscle following denervation.
  • Assessment of antioxidant drug efficacy in preventing denervation-induced muscle mass reduction.

Main Results:

  • Denervation induces oxidative stress in skeletal muscle.
  • Antioxidant treatments failed to prevent denervation-induced muscle mass reduction.
  • Oxidative stress contributes to the response to denervation but does not solely cause oxidative damage or atrophy.

Conclusions:

  • Oxidative stress is a consequence, not the primary driver, of denervation-induced skeletal muscle atrophy.
  • Targeting oxidative stress alone is insufficient to prevent neurogenic muscle atrophy.
  • Further research is needed to elucidate the complete molecular mechanisms of denervation-induced muscle atrophy.