Related Experiment Video
Updated: Jun 30, 2026
10:10
Preparation and Culture of Myogenic Precursor Cells/Primary Myoblasts from Skeletal Muscle of Adult and Aged Humans
Published on: February 16, 2017
Extracellular Vesicles From Young Human Myogenic Progenitor Cells Rejuvenate Aged Cells
Lorenzo Marramiero1,2,3, Ester Sara Di Filippo1,2,3, Federica Di Marco1
1Dept Neuroscience, Imaging and Clinical Sciences, "G. d'Annunzio" University Chieti-Pescara, 66100, Chieti, Italy, unich.it.
Biochemistry Research International
|June 29, 2026
Summary
Young muscle stem cell-derived extracellular vesicles (EVs) possess regenerative signals that combat age-related muscle decline. Elderly EVs impair younger cells, highlighting age-related changes in these crucial cell communicators.
Area of Science:
- Cell Biology
- Gerontology
- Biochemistry
Background:
- Skeletal muscle aging leads to loss of mass, power, and function.
- Skeletal muscle is a secretory organ, with myogenic progenitor cells (hMPCs) releasing extracellular vesicles (EVs).
- The role of hMPC-derived EVs in skeletal muscle aging requires further investigation.
Purpose of the Study:
- To investigate the role of hMPC-derived EVs in mediating skeletal muscle aging.
- To analyze age-related variations in EV cargo and their impact on human muscle stem cell function.
Main Methods:
- Isolation and characterization of EVs from young and elderly hMPCs.
- Analysis of EV miRNA and proteomic profiles.
- Treatment of elderly hMPCs with young EVs and vice versa to assess viability and differentiation.
Main Results:
- EVs from young hMPCs contain regenerative signals that improve aged muscle stem cell function.
- EVs from elderly hMPCs impair the regenerative capacity of younger muscle stem cells.
- hMPC-derived EVs modulate hMPC viability and differentiation, with age-dependent cargo.
Conclusions:
- hMPC-derived EVs play a significant role in skeletal muscle aging.
- The cargo of EVs is modulated by donor age, impacting muscle stem cell function.
- Targeting EVs may offer therapeutic strategies for age-related muscle decline.
Keywords:
extracellular vesicleshuman adult myogenic progenitor cellsmicroRNAssenescenceskeletal muscleMore Related Videos
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