Related Experiment Video
Updated: Mar 3, 2026

Metabolomic Analysis of Barley by Gas Chromatography/Mass Spectrometry
Published on: November 8, 2024
Reducing Stomatal Density in Barley Improves Drought Tolerance without Impacting on Yield
Jon Hughes1,2,3, Christopher Hepworth1,2,3, Chris Dutton1,2,3
1Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, United Kingdom (J.H., C.D., J.A.D., L.H., J.E.G.).
Abstract:
The epidermal patterning factor (EPF) family of secreted signaling peptides regulate the frequency of stomatal development in model dicot and basal land plant species. Here, we identify and manipulate the expression of a barley (Hordeum vulgare) ortholog and demonstrate that when overexpressed HvEPF1 limits entry to, and progression through, the stomatal development pathway. Despite substantial reductions in leaf gas exchange, barley plants with significantly reduced stomatal density show no reductions in grain yield. In addition, HvEPF1OE barley lines exhibit significantly enhanced water use efficiency, drought tolerance, and soil water conservation properties. Our results demonstrate the potential of manipulating stomatal frequency for the protection and optimization of cereal crop yields under future drier environments.
Related Concept Videos
Adaptations that Reduce Water Loss
Responses to Drought and Flooding
Regulation of Transpiration by Stomata
Responses to Salt Stress
Responses to Heat and Cold Stress

