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Updated: Mar 3, 2026
A Quantitative Assessment of The Yeast Lipidome using Electrospray Ionization Mass Spectrometry
Published on: August 21, 2009
Quantitative Analysis of Yeast Phospholipids and Sterols by High-Performance Liquid Chromatography-Evaporative
Oskar L Knittelfelder1, Sepp D Kohlwein2
1Institute of Molecular Biosciences, University of Graz, BioTechMed Graz, 8010 Graz, Austria.
Abstract:
Normal-phase high-performance liquid chromatography (HPLC) is a standard method for separating the major lipid classes in an extract. Owing to the absence of a common property like light absorbance in the various lipid classes, evaporative light-scattering detection (ELSD) is the method of choice for qualitative and quantitative lipid detection. In most cases, neutral lipids and polar lipids are separated by different solvent systems, making it necessary to perform multiple analyses. Compared with other techniques like thin-layer chromatography, normal-phase HPLC-ELSD has better reproducibility and allows a higher degree of automation. Here we describe a method for separating and quantifying yeast neutral lipids and glycerophospholipids in one analytical run.
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