Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ordered processing and splicing in a polycistronic transcript in liverwort chloroplasts.

T Kohchi1, Y Ogura, K Umesono

  • 1Research Center for Cell and Tissue Culture, Faculty of Agriculture, Kyoto University, Japan.

Current Genetics
|August 1, 1988
PubMed
Summary

Researchers identified ORF203, a split gene in Marchantia polymorpha chloroplast DNA, revealing ordered RNA processing in land plants. Its gene product may associate with chloroplast membranes.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Time-to-onset of diabetes with everolimus use: analysis of a spontaneous reporting system database.

Die Pharmazie·2021
Same author

Investigation of immune complexes formed by mitochondrial antigens containing a new lipoylated site in sera of primary biliary cholangitis patients.

Clinical and experimental immunology·2021
Same author

Immune complexome analysis reveals the presence of immune complexes and identifies disease-specific immune complex antigens in saliva samples from patients with Sjögren's syndrome.

Clinical and experimental immunology·2021
Same author

Psoriasis associated with ACE inhibitors: an analysis of the FAERS database.

Die Pharmazie·2020
Same author

Evaluation of syncope association with α<sub>1</sub>-adrenoceptor blockers in males using the FAERS database: impact of concomitant hypertension.

Die Pharmazie·2020
Same author

Proteomic profile of circulating immune complexes in chronic Chagas disease.

Parasite immunology·2016

Area of Science:

  • * Plant Molecular Biology
  • * Organelle Genetics
  • * Evolutionary Biology

Background:

  • * The complete chloroplast DNA sequence of the liverwort Marchantia polymorpha was analyzed.
  • * An unidentified open reading frame, designated ORF203, was discovered between the psbB and rps12' genes.

Purpose of the Study:

  • * To characterize the structure and expression of the novel ORF203.
  • * To investigate the RNA processing mechanisms in Marchantia polymorpha chloroplasts.

Main Methods:

  • * Northern blot analysis to detect multiple transcripts.
  • * S1 nuclease mapping to determine the transcription initiation site.
  • * Oligodeoxyribonucleotide probes for spliced RNA identification.
  • * Computer-based trans-membrane analysis.

Related Experiment Videos

Main Results:

  • * ORF203 was identified as a split gene with three exons and two group II introns.
  • * Multiple transcripts of ORF203 were detected.
  • * Co-transcription with downstream genes (rps12', rpl20) followed by processing into a precursor.
  • * Transcription initiated 52 nucleotides upstream of the coding sequence.
  • * Splicing occurred sequentially from 5' to 3' direction.
  • * Trans-membrane analysis suggested potential chloroplast membrane association of the ORF203 product.

Conclusions:

  • * Ordered RNA processing occurs in land plant chloroplasts.
  • * ORF203 represents a novel chloroplast gene with potential membrane functions.