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Ageing of fixed cytological preparations produces degradation of chromosomal DNA
R Mezzanotte1, R Vanni, O Flore
1Istituto di Biologia Generale, Facolta di Medicina e Chirurgia, Universita di Cagliari, Italy.
Cytogenetics and Cell Genetics
|January 1, 1988
Abstract:
When fixed chromosome preparations were allowed to age for 1-72 h, they became progressively more susceptible to digestion by exonuclease III and by S1 nuclease. Analysis of DNA from these aged preparations on agarose gels showed that the molecular weight of the DNA decreased as ageing progressed. We conclude that DNA in fixed chromosome preparations becomes progressively degraded as the preparations age.