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Meniscal Tissue Engineering Using Aligned Collagen Fibrous Scaffolds: Comparison of Different Human Cell Sources
Jihye Baek1,2, Sujata Sovani1, Wonchul Choi1
11 Shiley Center for Orthopaedic Research and Education at Scripps Clinic , La Jolla, California.
Tissue Engineering. Part A
|May 3, 2017
Summary
Infrapatellar fat pad cells show promise for meniscus regeneration, forming meniscus-like tissues on collagen scaffolds. These cells exhibit superior gene expression and mechanical properties compared to other sources, supporting cell-based tissue engineering strategies.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Regenerative Medicine
Background:
- Hydrogel and electrospun scaffolds mimic native extracellular matrix for neotissue development.
- Investigating diverse human cell sources is crucial for effective tissue regeneration strategies.
Purpose of the Study:
- To evaluate meniscus tissue generation using various human cell types on electrospun collagen scaffolds.
- To assess the potential of infrapatellar fat pad (IPFP) cells for meniscus regeneration.
Main Methods:
- Seeding cells (meniscus, mesenchymal stem cells, synovial, IPFP) onto aligned electrospun collagen type I scaffolds, with optional hydrogel encapsulation.
- Culturing constructs with growth factors (TGFβ1, TGFβ3) and analyzing cell viability, morphology, gene expression (qPCR), histology, and mechanical properties.
- Comparing neotissue formation and characteristics across different cell types.
Main Results:
- All cell types showed increased collagen type I (COL1A1) and other matrix gene expression with TGFβ1/TGFβ3 treatment.
- Infrapatellar fat pad (IPFP) cells demonstrated superior meniscogenic gene expression, mechanical properties, and cell distribution.
- Electrospun collagen scaffolds supported neotissue formation across all tested cell types.
Conclusions:
- Electrospun collagen scaffolds effectively support neotissue formation.
- Infrapatellar fat pad (IPFP) cells hold significant potential for cell-based meniscus regeneration strategies due to their superior performance.
