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Gastrin receptors in human gastric scirrhous carcinoma
1First Department of Internal Medicine, School of Medicine, Hiroshima University, Japan.
Abstract:
Specific binding of 125I-labeled gastrin-17 in tumor tissues and gastric mucosa of 11 advanced human gastric carcinoma cases were studied by single-point receptor assay, using 400 pM 125I-labeled gastrin-17. Membrane preparations (800-15,000 g fraction) of the tissues were examined in this study. In 4 of the 5 cases of gastric scirrhous carcinoma (Borrmann type IV), the tumor tissues showed a high degree of specific bindings (1.1-18.2 femtomoles per milligram protein), but in 5 of the 6 cases of Borrmann type II or III, no specific bindings were detected. These findings indicate that gastrin receptor is frequently present in human gastric scirrhous carcinoma.
Insights
Gastrin receptors are frequently found in advanced gastric scirrhous carcinoma (Borrmann type IV) but not in Borrmann types II or III. This suggests a potential role for gastrin in scirrhous gastric tumor development.
Area of Science:
- Oncology
- Gastroenterology
- Molecular Biology
Background:
- Gastric carcinoma is a significant global health concern.
- Gastrin and its receptor play roles in gastric physiology and potentially in tumor growth.
Purpose of the Study:
- To investigate the presence and levels of gastrin receptors in advanced human gastric carcinoma tissues.
- To determine if gastrin receptor expression differs between gastric scirrhous carcinoma (Borrmann type IV) and other types (Borrmann types II/III).
Main Methods:
- Utilized a single-point receptor assay with 125I-labeled gastrin-17.
- Analyzed membrane preparations from tumor tissues and gastric mucosa of 11 advanced gastric carcinoma patients.
- Quantified specific binding of labeled gastrin-17.
Main Results:
- High specific binding of gastrin-17 was detected in 4 out of 5 gastric scirrhous carcinoma (Borrmann type IV) cases (1.1-18.2 fmol/mg protein).
- No specific binding was observed in 5 out of 6 cases of Borrmann type II or III gastric carcinoma.
- Gastrin receptor expression was significantly associated with gastric scirrhous carcinoma.
Conclusions:
- Gastrin receptors are frequently present in human gastric scirrhous carcinoma.
- The findings suggest a potential link between gastrin signaling and the pathogenesis of gastric scirrhous carcinoma.
- Further research into gastrin receptor targeted therapies for scirrhous gastric cancer is warranted.