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Updated: Mar 3, 2026
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Sampling and Pretreatment of Tooth Enamel Carbonate for Stable Carbon and Oxygen Isotope Analysis
Published on: August 15, 2018
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Enamel Mineral Content Changes After Bleaching With High and Low Hydrogen Peroxide Concentrations: Colorimetric
Operative Dentistry
|May 4, 2017
Summary
Hydrogen peroxide (HP) bleaching agents did not significantly alter calcium and phosphorous levels in tooth enamel, regardless of concentration. Both high and low concentration HP treatments showed no correlation between measurement methods for mineral content changes.
Area of Science:
- Dental Materials Science
- Biomineralization
- Analytical Chemistry
Background:
- Dental bleaching with hydrogen peroxide (HP) is a common cosmetic procedure.
- Concerns exist regarding the potential effects of HP on tooth enamel's mineral content.
- Understanding the impact of varying HP concentrations on calcium (Ca) and phosphorous (P) is crucial for safety and efficacy.
Purpose of the Study:
- To evaluate the Ca and P content in human enamel after bleaching with high and low concentrations of HP.
- To compare the effectiveness of Total Reflection X-Ray Fluorescence (TXRF) and colorimetric spectrophotometry (SPEC) in assessing mineral changes.
- To determine if HP concentration influences enamel demineralization during bleaching.
Main Methods:
- Human third molars were treated with various in-office (high HP) and at-home (low HP) bleaching agents.
- Enamel samples were analyzed for Ca and P content using TXRF and SPEC at baseline, during, and after treatment.
- Statistical analyses (Kruskal-Wallis, Spearman) were performed to assess mineral content changes and method correlation.
Main Results:
- TXRF analysis indicated no significant influence of bleaching agents on Ca and P content (p>0.05).
- SPEC analysis revealed a decrease in Ca over time for several agents and a significant decrease in P for two agents.
- No significant correlation was found between Ca and P values obtained by SPEC and TXRF (p>0.05).
Conclusions:
- Changes in enamel Ca and P content during HP bleaching occurred independently of HP concentration.
- TXRF and SPEC methods showed no correlation in detecting mineral changes, suggesting differing sensitivities or mechanisms.
- Further research is needed to clarify the discrepancies between analytical methods and the clinical significance of minor mineral fluctuations.
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