Related Experiment Video
Updated: Mar 3, 2026
08:07
Analyzing DNA-Protein Interactions with Streptavidin-Based Biolayer Interferometry
Published on: January 17, 2025
2.3K
Dpb11 may function with RPA and DNA to initiate DNA replication
Irina Bruck1, Nalini Dhingra1, Matthew P Martinez1
1Florida State University College of Medicine, Department of Biomedical Sciences, Tallahassee, Florida, United States of America.
Plos One
|May 4, 2017
Summary
Dpb11 protein is crucial for DNA replication initiation. It binds to DNA and helps recruit RPA, stabilizing DNA origins for replication.
Area of Science:
- Molecular Biology
- Cell Biology
- Biochemistry
Background:
- DNA replication initiation is a complex process.
- Dpb11 is a key protein in budding yeast DNA replication.
- Understanding Dpb11's function is vital for cell cycle control.
Purpose of the Study:
- To investigate the role of Dpb11 in DNA replication initiation.
- To characterize the DNA-binding properties of Dpb11 and its interaction with RPA.
- To elucidate the mechanism by which Dpb11 facilitates origin melting and stabilization.
Main Methods:
- Biochemical assays to study protein-DNA interactions.
- Analysis of Dpb11 mutants in budding yeast.
- Assessment of protein recruitment to replication origins using molecular biology techniques.
Main Results:
- Dpb11 binds tightly to single-stranded and branched DNA structures.
- Dpb11 forms a stable complex with CDK-phosphorylated RPA in the presence of branched DNA.
- A Dpb11 mutant defective in DNA binding shows reduced RPA recruitment to origins and diminished GINS interaction with Mcm2-7.
Conclusions:
- Dpb11-DNA interaction is essential for RPA recruitment to origins.
- The interaction between Dpb11, phosphorylated RPA, and branched DNA stabilizes melted origin DNA.
- Dpb11 may also play a role in helicase activation during DNA replication initiation.
Related Concept Videos
02:37Restarting Stalled Replication Forks
6.5K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
6.5K
02:31Homologous Recombination
64.4K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
64.4K
01:03Single-Strand DNA Binding Proteins
17.0K
For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
17.0K
02:36DNA Damage can Stall the Cell Cycle
10.3K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
10.3K
02:36DNA Damage Can Stall the Cell Cycle
3.3K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
3.3K
03:01The Replisome
38.8K
DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
38.8K