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Updated: Mar 3, 2026

Semi-automated Biopanning of Bacterial Display Libraries for Peptide Affinity Reagent Discovery and Analysis of Resulting Isolates
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Two epitopes responsible for the catalytic activity of heme oxygenase-1 identified by phage display

Xuran Wei1, Qingjun Liu1, Yaping Gao2

  • 1Beijing Key Laboratory of Blood Safety and Supply Technologies Beijing Institute of Transfusion Medicine China.

FEBS Open Bio
|May 5, 2017
PubMed

Heme oxygenase-1 (HO-1) catalyzes the oxidative degradation of heme. The catalytic mechanism of the HO-1 reaction has been determined gradually by studies of its crystal structure and HO-1 mutants. However, the neutralizing epitopes responsible for HO-1 activity remain elusive. Screening of a phage display library revealed four epitopes that could interact with the polyclonal antibody prepared by immunizing rabbits with the purified HO-1 protein. Two of these four epitopes are responsible for HO-1 catalytic activity because their antibodies were able to neutralize HO-1 activity. The results of the present study shed further light on the molecular character of HO-1.

Keywords:
catalytic activity of HO‐1epitopeheme oxygenase‐1phage display

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