Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Corrigendum to "Scientific Opinion on Biphenyl-2-ol and Sodium 2-biphenylolate used in cosmetic products (CAS/EC No. 90-43-7/201-993-5 and 132-27-4/205-055-6)- SCCS/1669/24" [NAM Journal Volume 1, 2025, 100035].

NAM journal·2026
Same author

<i>In vitro</i> models for gonadal and placental toxicity: A review and industry survey.

NAM journal·2026
Same author

SCCS opinion on biphenyl-2-ol and sodium 2-biphenylolate used in cosmetic products (CAS/EC No. 90-43-7/201-993-5 and 132-27-4/205-055-6)- SCCS/1669/24.

NAM journal·2026
Same author

Interleukin-19 ameliorates drug-induced liver injury by limiting proinflammatory macrophage infiltration via SUMOylation of C/EBPβ.

Journal of hepatology·2026
Same author

A combined in silico and in vitro new approach methodology for early detection of liver steatogenic chemicals.

Chemico-biological interactions·2026
Same author

Towards advanced in vitro models of testicular steroidogenesis for endocrine disruption testing.

ALTEX·2026
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Mar 3, 2026

Viability Assays for Cells in Culture
12:03

Viability Assays for Cells in Culture

Published on: January 20, 2014

47.4K

Assaying Cellular Viability Using the Neutral Red Uptake Assay.

Gamze Ates1, Tamara Vanhaecke1, Vera Rogiers1

  • 1Department of In Vitro Toxicology and Dermato-Cosmetology, Faculty of Medicine and Pharmacy, Vrije Universiteit Brussel, Brussels, Belgium.

Methods in Molecular Biology (Clifton, N.J.)
|May 5, 2017
PubMed
Summary
This summary is machine-generated.

The neutral red uptake assay quantifies cell viability and chemical toxicity by measuring dye uptake in lysosomes. This in vitro toxicology method, using HepG2 cells, assesses cytotoxicity from compounds like acetaminophen.

Keywords:
HepG2Neutral red uptakeViability assay

More Related Videos

An Automated Differential Nuclear Staining Assay for Accurate Determination of Mitocan Cytotoxicity
07:58

An Automated Differential Nuclear Staining Assay for Accurate Determination of Mitocan Cytotoxicity

Published on: May 12, 2020

7.5K
Evaluating Primary Blast Effects In Vitro
10:51

Evaluating Primary Blast Effects In Vitro

Published on: September 18, 2017

8.5K

Related Experiment Videos

Last Updated: Mar 3, 2026

Viability Assays for Cells in Culture
12:03

Viability Assays for Cells in Culture

Published on: January 20, 2014

47.4K
An Automated Differential Nuclear Staining Assay for Accurate Determination of Mitocan Cytotoxicity
07:58

An Automated Differential Nuclear Staining Assay for Accurate Determination of Mitocan Cytotoxicity

Published on: May 12, 2020

7.5K
Evaluating Primary Blast Effects In Vitro
10:51

Evaluating Primary Blast Effects In Vitro

Published on: September 18, 2017

8.5K

Area of Science:

  • Toxicology
  • Cell Biology
  • Biochemistry

Background:

  • The neutral red uptake assay is a vital tool for in vitro cytotoxicity assessment.
  • It quantifies xenobiotic-induced toxicity by measuring neutral red dye uptake in cell lysosomes.
  • This assay is crucial for hazard identification in toxicological studies.

Purpose of the Study:

  • To detail a protocol for the neutral red uptake assay using the HepG2 human hepatoma cell line.
  • To demonstrate the assay's utility in assessing the cytotoxicity of specific xenobiotics.
  • To provide an alternative in vitro model for human hepatocytes in toxicological evaluations.

Main Methods:

  • Utilizing the neutral red uptake assay with the HepG2 cell line.
  • Exposing cells to varying concentrations of xenobiotics (acetaminophen and acetyl salicylic acid).
  • Quantifying cytotoxicity based on the concentration-dependent reduction of neutral red dye uptake.

Main Results:

  • The study successfully applied the neutral red uptake assay to HepG2 cells.
  • Cytotoxicity of acetaminophen and acetyl salicylic acid was assessed using this method.
  • The assay demonstrated its capability to quantify chemical-induced cell damage.

Conclusions:

  • The neutral red uptake assay provides a reliable method for in vitro cytotoxicity testing.
  • HepG2 cells serve as a suitable model for assessing human hepatocyte responses to xenobiotics.
  • This assay contributes to the regulatory acceptance of in vitro toxicology methods, such as the 3T3-NRU-phototoxicity assay (OECD TG 432).