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High-Throughput Production of Proteins in E. coli for Structural Studies
Charikleia Black1, John J Barker2, Richard B Hitchman1
1Evotec Ltd, 114 Innovation Drive, Milton Park, Abingdon, Oxfordshire, OX14 4RZ, UK.
Methods in Molecular Biology (Clifton, N.J.)
|May 5, 2017
Abstract:
We have developed a standardized and efficient workflow for high-throughput (HT) protein expression in E. coli and parallel purification which can be tailored to the downstream application of the target proteins. It includes a one-step purification for the purposes of functional assays and a two-step protocol for crystallographic studies, with the option of on-column tag removal.
