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Updated: Mar 3, 2026

Visualization of Endosome Dynamics in Living Nerve Terminals with Four-dimensional Fluorescence Imaging
Published on: April 16, 2014
Improving your four-dimensional image: traveling through a decade of light-sheet-based fluorescence microscopy
Frederic Strobl1, Alexander Schmitz1, Ernst H K Stelzer1
1Physical Biology/Physikalische Biologie (IZN, FB 15), Buchmann Institute for Molecular Life Sciences (BMLS), Cluster of Excellence Frankfurt-Macromolecular Complexes (CEF-MC), Goethe Universität-Frankfurt am Main (Campus Riedberg), Frankfurt am Main, Germany.
Abstract:
Light-sheet-based fluorescence microscopy features optical sectioning in the excitation process. This reduces phototoxicity and photobleaching by up to four orders of magnitude compared with that caused by confocal fluorescence microscopy, simplifies segmentation and quantification for three-dimensional cell biology, and supports the transition from on-demand to systematic data acquisition in developmental biology applications.
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