Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Decrease of topoisomerase I activity in the testes of infertile men with varicocele.

M Fujisawa1, S Yoshida, O Matsumoto

  • 1Department of Urology, Kobe University School of Medicine, Japan.

Archives of Andrology
|January 1, 1988
PubMed
Summary

Infertile men with varicocele often have lower topoisomerase I activity, an enzyme crucial for DNA metabolism. This reduction may contribute to reduced sperm production in affected individuals.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Anti-eye muscle antibodies and hypothyroid Graves' disease: a case report.

Endocrinologia japonica·1992
Same author

[Irritable bowel syndrome in children].

Nihon rinsho. Japanese journal of clinical medicine·1992
Same author

Carbohydrate-binding proteins in bovine kidney have consensus amino acid sequences of annexin family proteins.

The Journal of biological chemistry·1992
Same author

Efficient purification of a full length and biochemically active p110Rb, the retinoblastoma gene product.

Biochemical and biophysical research communications·1992
Same author

[MR imaging of thymoma--comparison with CT, operative, and pathological findings].

Nihon Igaku Hoshasen Gakkai zasshi. Nippon acta radiologica·1992
Same author

Photostability and biological activity of fluoroquinolones substituted at the 8 position after UV irradiation.

Antimicrobial agents and chemotherapy·1992

Area of Science:

  • Reproductive Biology
  • Molecular Biology
  • Genetics

Background:

  • Topoisomerase I is essential for DNA replication and repair.
  • Varicocele is a common cause of male infertility.
  • The role of topoisomerase I in varicocele-associated infertility is not well understood.

Purpose of the Study:

  • To investigate topoisomerase I activity in the testes of infertile men with varicocele.
  • To determine the correlation between varicocele grade and topoisomerase I levels.
  • To explore the relationship between topoisomerase I activity and sperm quality.

Main Methods:

  • Measurement of topoisomerase I activity in testicular biopsy specimens from 37 infertile men with varicocele.
  • Comparison of enzyme activity between infertile men and normal controls.

Related Experiment Videos

  • Correlation analysis with varicocele grade and Johnsen's sperm morphology score.

    • Main Results:

    • 38% of infertile men with varicocele exhibited reduced topoisomerase I activity compared to normal men.
    • Reduced topoisomerase I activity was more prevalent in high-grade (grade III) varicocele (46%) than in low-grade (grades I and II) varicocele (26%).
    • Patients with lower topoisomerase I activity had significantly lower Johnsen's scores, indicating poorer sperm quality.

    Conclusions:

    • Decreased topoisomerase I activity is observed in a significant proportion of infertile men with varicocele.
    • The reduction in topoisomerase I activity appears to be associated with varicocele severity.
    • Reduced topoisomerase I may play a role in the hypospermatogenesis observed in varicocele-associated infertility.