Related Experiment Video
Updated: Mar 3, 2026

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants
Published on: June 6, 2025
Phospho-STAT5B Expression Is a Prognostic Marker for Merkel Cell Carcinoma
Taku Fujimura1, Sadanori Furudate2, Yumi Kambayahsi2
1Department of Dermatology, Tohoku University Graduate School of Medicine, Sendai, Japan tfujimura1@mac.com.
Phospho-signal transducer and activator of transcription 5B (pSTAT5B) expression is a promising prognostic marker for Merkel cell carcinoma (MCC). This finding offers a clearer method for evaluating outcomes in MCC patients, aiding in treatment decisions.
Area of Science:
- Oncology
- Immunology
- Dermatology
Background:
- Merkel cell carcinoma (MCC) is an aggressive skin cancer.
- Tumor-infiltrating leukocytes (TILs), particularly CD8+ T-cells, are implicated in MCC development.
- Current methods for assessing prognostic factors in MCC are limited.
Purpose of the Study:
- To identify reliable prognostic factors for Merkel cell carcinoma (MCC).
- To evaluate phospho-signal transducer and activator of transcription 5B (pSTAT5B) as a prognostic marker in MCC.
- To explore other potential stromal and immune-related prognostic markers.
Main Methods:
- Immunohistochemical staining was performed on 24 MCC patient samples.
- pSTAT5B expression was assessed as a potential prognostic marker.
- Expression of periostin (POSTN), interleukin-4, and various TIL subsets were also evaluated.
Main Results:
- All 16 MCC cases with a good outcome expressed pSTAT5B.
- All 8 MCC cases with a poor outcome lacked pSTAT5B expression.
- No significant differences in stromal factors were observed between good and poor outcome groups.
Conclusions:
- pSTAT5B expression is a potential indicator of a positive prognosis in Merkel cell carcinoma (MCC).
- This marker may provide a more accessible method for prognostic evaluation in MCC.
More Related Videos
08:38Phospho Flow Cytometry with Fluorescent Cell Barcoding for Single Cell Signaling Analysis and Biomarker Discovery
Published on: October 4, 2018
07:12Time-resolved Förster Resonance Energy Transfer Assays for Measurement of Endogenous Phosphorylated STAT Proteins in Human Cells
Published on: September 9, 2021
Related Concept Videos
PI3K/mTOR/AKT Signaling Pathway
mTOR Signaling and Cancer Progression
The mTOR pathway or the...
Interactions Between Signaling Pathways
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Protein Kinases and Phosphatases
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
The JAK-STAT Signaling Pathway
MAPK Signaling Cascades