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Engineered Bacillus pumilus laccase-like multi-copper oxidase for enhanced oxidation of the lignin model compound
Julian Ihssen1, Dagmara Jankowska2, Thomas Ramsauer2
1Laboratory for Biomaterials, Empa, Swiss Federal Laboratories for Materials Science and Technology, Lerchenfeldstrasse 5, 9014 St. Gallen, CH, Switzerland.
Abstract:
Laccases and laccase-like multi-copper oxidases (LMCOs) are versatile and robust biocatalysts applied in a variety of oxidative processes, and various studies have attempted to improve their catalytic activity. Here we report the engineering of a bacterial LMCO for enhanced oxidation of the lignin-related compound guaiacol by a combination of structure-guided mutagenesis and DNA shuffling. Mutant L9 showed a 1.39 mM Km for guaiacol and a 2.5-fold increase in turnover rate (kcat/Km = 2.85·104 M-1s-1).
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