Related Experiment Video
Updated: Mar 2, 2026
06:36
Improved Enzyme Protection Assay to Study Staphylococcus aureus Internalization and Intracellular Efficacy of Antimicrobial Compounds
Published on: September 8, 2021
3.3K
The Surface Protein SdrF Mediates Staphylococcus epidermidis Adherence to Keratin
Sheetal Trivedi1, Anne-Catrin Uhlemann1, Philippe Herman-Bausier2
11Division of Infectious Diseases.
The Journal of Infectious Diseases
|May 9, 2017
Summary
Staphylococcus epidermidis surface protein SdrF adheres to skin keratin, facilitating bacterial colonization. This study reveals SdrF
Area of Science:
- Microbiology
- Dermatology
- Biochemistry
Background:
- Staphylococcus epidermidis is a common skin bacterium and opportunistic pathogen.
- Staphylococcal surface proteins mediate host tissue attachment, aiding pathogenesis.
- SdrF is a surface protein implicated in staphylococcal adhesion.
Purpose of the Study:
- To investigate the adherence of Staphylococcus epidermidis surface protein SdrF to keratin.
- To understand the role of SdrF in S. epidermidis skin colonization.
Main Methods:
- Expressing SdrF and its domains in Lactococcus lactis.
- Assessing adherence to keratin types 1 and 10, keratinocytes, and epithelial cells.
- Utilizing anti-SdrF antibodies and RNA interference to modulate adherence.
- Employing atomic force microscopy for direct force measurements.
Main Results:
- SdrF demonstrated binding to human keratins 1 and 10, and adhered to keratinocytes and epithelial cells.
- Adherence involved both A and B domains of SdrF, with strong adhesion mediated by the A region.
- Antibodies against SdrF and reduced keratin synthesis decreased bacterial adherence.
Conclusions:
- SdrF mediates Staphylococcus epidermidis adherence to human keratin.
- SdrF likely plays a role in the colonization of S. epidermidis on the skin.
Related Concept Videos
01:24Adherens Junctions
7.0K
Strong contact points between adjacent cells anchor them to each other, forming tissues. Such anchoring junctions are of two types – adherens junctions and desmosomes. Adherens junctions are abundant in tissues such as epithelium and endothelium, forming a continuous zone of adhesion called the adhesion belt. In other tissues, such as heart muscle, they appear as clusters, linking the cells to produce coordinated heart muscle contraction.
Adherens Junctions are Dynamic
Adherens Junctions are Dynamic
7.0K
01:23Surface Appendages of Archaea
764
Archaeal surface appendages are highly specialized structures essential for environmental adaptation, encompassing roles in adhesion, biofilm formation, and motility. Among these appendages, pili and archaella stand out for their distinct morphologies and functionalities, enabling archaea to thrive in diverse and often extreme environments.Pili: Adhesion and Biofilm FormationPili are filamentous structures assembled from pilin protein subunits, primarily contributing to adhesion and biofilm...
764
01:03Anchoring Junctions
5.4K
Anchoring junctions are multiprotein complexes that help cells connect to other cells and the extracellular matrix. Anchoring junctions are present on the lateral and basal surfaces of cells, providing strong and flexible connections. Focal adhesions are often formed due to cell interactions with the ECM substrata, which initiate signal transduction via kinase cascades and other mechanisms. Together, they provide stability and tissue integrity. There are three types of anchoring junctions:...
5.4K
01:05Desmosomes
8.3K
The term desmosome derives from the Greek words "desmo" and "soma" meaning "adhesion bodies." This structure was first observed during the late 1800s and described as small, dense nodules in the epidermis. Desmosomes are button-like structures that help form an interlinked network of intermediate filaments across the cells. These junctions are essential to hold cells together under mechanical stress and to maintain tissue integrity. Desmosomes are multi-protein...
8.3K
01:17Intracellular Signaling Affects Focal Adhesions
3.7K
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
Some...
3.7K
01:28Types of Membrane Protrusions
3.8K
The protrusion of the cell surface is an initial step for several cellular processes, including cell migration, phagocytosis, and neurite outgrowth. These membrane protrusions are a result of cytoskeletal rearrangement. The most widely observed cell protrusions include lamellipodia, pseudopodia, filopodia, microvilli, invadopodia, and podosomes. These protrusions can be of two types — static or dynamic.
The microvilli, an example of stable protrusions, are finger-like projections...
The microvilli, an example of stable protrusions, are finger-like projections...
3.8K