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Interlinked Macroporous 3D Scaffolds from Microgel Rods
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Clickable Microgel Scaffolds as Platforms for 3D Cell Encapsulation.

Alexander S Caldwell1, Gavin T Campbell1, Kelly M T Shekiro1

  • 1Department of Chemical and Biological Engineering and the BioFrontiers Institute, University of Colorado Boulder, Jennie Smoly Caruthers Biotechnology Building, 3415 Colorado Ave, Boulder, CO, 80303, USA.

Advanced Healthcare Materials
|May 10, 2017
PubMed
Summary

Researchers developed a novel bottom-up method using microgel building blocks to create porous, cell-laden scaffolds for regenerative medicine. This technique allows for in situ cell incorporation and tunable microenvironments, influencing cell behavior.

Keywords:
bottom-up assemblyhMSCshydrogelsmicrogelsmicroporous networks

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Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Tissue Engineering

Background:

  • Microporous scaffolds are crucial for regenerative medicine and tissue repair.
  • Conventional fabrication methods (templating, top-down) have limitations.
  • Bottom-up assembly offers in situ cell incorporation and complex composite formation.

Purpose of the Study:

  • To synthesize microgel building blocks for bottom-up fabrication of porous, cell-laden scaffolds.
  • To investigate the influence of tunable microenvironments on cell morphology and behavior.
  • To establish a versatile platform for recapitulating biological interfaces.

Main Methods:

  • Synthesis of microgel building blocks with clickable surface groups.
  • Bottom-up assembly of microgels to form porous scaffolds.
  • Incorporation of human mesenchymal stem cells during scaffold fabrication.
  • Tuning particle size (≈10 and 100 µm) to create varied microenvironments.

Main Results:

  • Successful fabrication of porous, cell-laden scaffolds using a bottom-up approach.
  • Demonstrated incorporation of human mesenchymal stem cells within the scaffolds.
  • Showcased how varying pore sizes and distributions significantly alter cell morphology and cytoskeletal formation.
  • Highlighted the tunable properties of the microgel system for controlling cellular development.

Conclusions:

  • The developed microgel-based system enables bottom-up fabrication of tunable, cell-laden porous scaffolds.
  • This approach offers precise control over cellular microenvironments, impacting cell morphology and cytoskeletal organization.
  • The platform holds potential for advancing regenerative medicine and creating complex biomimetic interfaces.