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YY1 directly suppresses MYCT1 leading to laryngeal tumorigenesis and progress

Si-Yao Qu1,2, Yuan-Yuan Sun1, Yun-Hui Li3

  • 1Department of Medical Genetics, China Medical University, Shenyang, 110122, China.

Cancer Medicine
|May 10, 2017
PubMed

Insights

The transcription factor YY1 promotes laryngeal cancer progression by inhibiting MYCT1 expression, increasing cell proliferation and migration while suppressing apoptosis. This suggests YY1 is a potential oncogene target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Genetics

Background:

  • The transcription factor YY1 (Yin Yang 1) has diverse roles in various cancers, but its specific function in laryngeal cancer remains unclear.
  • Understanding the molecular mechanisms underlying laryngeal cancer is crucial for developing effective therapeutic strategies.

Purpose of the Study:

  • To investigate the role and mechanism of YY1 in laryngeal cancer.
  • To determine the relationship between YY1 and MYCT1 expression in laryngeal cancer.
  • To explore the potential of YY1 as a therapeutic target.

Main Methods:

  • Quantitative real-time RT-PCR and Western Blotting were used to measure YY1 and MYCT1 mRNA and protein levels.
  • Bioinformatics, chromatin immunoprecipitation, and dual-luciferase assays were employed to study the binding of YY1 to the MYCT1 promoter and its activity.
  • Cell proliferation, migration, and apoptosis assays (cell viability, colony formation, scratch, Transwell, and flow cytometry) were performed to assess cellular functions.

Main Results:

  • YY1 was upregulated and MYCT1 was downregulated in laryngeal cancer tissues, exhibiting a negative correlation.
  • Higher YY1 and lower MYCT1 expression correlated with lymphatic metastasis in laryngeal cancer patients.
  • YY1 directly bound to the MYCT1 promoter, inhibiting its activity, and YY1 knockdown reversed the effects of YY1 silence on cancer cell behavior.

Conclusions:

  • YY1 acts as an oncogene in laryngeal cancer by directly inhibiting MYCT1, thereby promoting proliferation and migration while suppressing apoptosis.
  • The YY1/MYCT1 axis represents a potential therapeutic target for laryngeal cancer treatment and progression management.
Keywords:
ApoptosisMYCT1YY1laryngeal cancermigrationproliferation

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