Related Experiment Video

Updated: Mar 2, 2026

All-optical Mechanobiology Interrogation of Yes-associated Protein in Human Cancer and Normal Cells using a Multi-functional System
09:55

All-optical Mechanobiology Interrogation of Yes-associated Protein in Human Cancer and Normal Cells using a Multi-functional System

Published on: December 20, 2021

3.4K

A TIAM Double Hit to Oppose YAP/TAZ

Luca Azzolin1, Stefano Piccolo1

  • 1Department of Molecular Medicine, University of Padua School of Medicine, viale Colombo 3, 35126 Padua, Italy.

Cancer Cell
|May 10, 2017
PubMed

Insights

TIAM1 inhibits colorectal cancer cell invasiveness through two mechanisms. It promotes TAZ degradation in the cytoplasm and blocks YAP/TAZ binding to TEAD in the nucleus, offering new therapeutic targets.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • Colorectal cancer (CRC) is a major global health concern.
  • Cancer cell invasiveness is a key factor in metastasis and poor prognosis.
  • Yeast and TAZ (TAZ) are transcriptional co-activators involved in cell proliferation and cancer progression.

Purpose of the Study:

  • To investigate the role of TIAM1 in regulating YAP/TAZ activity.
  • To elucidate the mechanisms by which TIAM1 affects colorectal cancer cell invasiveness.

Main Methods:

  • The study utilized cell culture models of colorectal cancer.
  • Techniques included co-immunoprecipitation, Western blotting, and immunofluorescence.
  • Functional assays assessed cell invasion and migration.

Main Results:

  • TIAM1 was found to interact with TAZ in the cytoplasm, promoting its degradation via the destruction complex.
  • TIAM1 was also shown to antagonize the binding of TAZ/YAP to the TEAD transcription factor in the nucleus.
  • These dual actions of TIAM1 effectively inhibited the invasiveness of colorectal cancer cells.

Conclusions:

  • TIAM1 possesses dual inhibitory mechanisms against YAP/TAZ.
  • TIAM1's cytoplasmic and nuclear actions suppress colorectal cancer cell invasiveness.
  • Targeting TIAM1 may represent a novel therapeutic strategy for colorectal cancer.

More Related Videos

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter11:32

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter

Published on: March 27, 2020

7.5K
Culturing and Manipulation of O9-1 Neural Crest Cells08:32

Culturing and Manipulation of O9-1 Neural Crest Cells

Published on: October 9, 2018

11.2K

Related Concept Videos

Flail Chest-II01:26

Flail Chest-II

Managing flail chest, a condition characterized by a segment of the chest wall moving independently from the rest of the thoracic cage, requires a comprehensive approach. It includes a thorough assessment of the patient's condition, a diagnostic evaluation to determine the extent of the injury, and the implementation of appropriate medical interventions tailored to the individual's needs.
Assessment:
1. Clinical Evaluation:
History:
755
Flail Chest-I01:24

Flail Chest-I

Overview of Flail Chest
Flail chest is a severe and potentially life-threatening condition characterized by the fracture of three or more adjacent ribs in multiple places. It is most commonly caused by direct impacts and trauma, such as motor vehicle accidents or injuries from a steering wheel impact. It can also occur due to falls in elderly individuals with osteoporosis, or assaults involving sharp objects.
Pathophysiology
The pathophysiology of flail chest is complex, involving fractures of...
869
Torts II01:13

Torts II

Intentional torts in healthcare refer to deliberate actions that cause harm or infringe on the rights of others. Understanding these torts is crucial for healthcare professionals to avoid legal liabilities and maintain ethical standards in patient care.
1.1K
Comparing Experimental Results: Student's t-Test01:09

Comparing Experimental Results: Student's t-Test

The t-test is a statistical method used to compare the sample mean with a population mean or compare two means from two data sets. The test statistic is calculated from the standard deviation, mean, and number of measurements in the data set at a selected confidence interval and then compared to a table of critical values at this confidence level. If the test statistic is smaller than the critical value, the null hypothesis is accepted. In this case, we state that the difference between the...
6.2K
Drag Force and Terminal Speed01:18

Drag Force and Terminal Speed

An interesting force in everyday life is the force of drag on an object when it is moving in a fluid. Like friction, the drag force always opposes the motion of an object. Unlike simple friction, the drag force is proportional to some function of the velocity of the object in that fluid. This functionality is complicated and depends upon the shape of the object, its size, its velocity, and the fluid it is in. For most large objects, such as cyclists, cars, and baseballs, that are not moving too...
3.5K
The Y-to-Y Circuit01:19

The Y-to-Y Circuit

In a balanced four-wire wye-to-wye system, the arrangement involves wye-connected sinusoidal voltage sources and loads, connected through a neutral wire that links the neutral nodes of the source and load. The load impedance is connected across each phase of the load. The wye-connected source can be connected to the wye-connected load in four-wire and three-wire arrangements. A three-phase system is considered balanced when the load on each phase is equal, leading to uniform current flow and...
789