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Updated: Mar 2, 2026

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells
Published on: March 16, 2017
Isoxazole derivatives as new nitric oxide elicitors in plants
Anca Oancea1, Emilian Georgescu2, Florentina Georgescu3
1National Institute of Research and Development for Biological Sciences, Spl. Independentei 296, RO-060031 Bucharest, Romania.
Abstract:
Several 3,5-disubstituted isoxazoles were obtained in good yields by regiospecific 1,3-dipolar cycloaddition reactions between aromatic nitrile oxides, generated in situ from the corresponding hydroxyimidoyl chlorides, with non-symmetrical activated alkynes in the presence of catalytic amounts of copper(I) iodide. Effects of 3,5-disubstituted isoxazoles on nitric oxide and reactive oxygen species generation in Arabidopsis tissues was studied using specific diaminofluoresceine dyes as fluorescence indicators.
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