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Updated: Mar 2, 2026

Nanosensors to Detect Protease Activity In Vivo for Noninvasive Diagnostics

Nanosensors to Detect Protease Activity In Vivo for Noninvasive Diagnostics

Published on: July 16, 2018

Recent developments in protease activity assays and sensors.

Irvine Lian Hao Ong¹, Kun-Lin Yang¹

¹National University of Singapore, 4 Engineering Drive 4, Singapore 117585. cheyk@nus.edu.sg.

|May 11, 2017

Summary

Protease activity assays are crucial for disease biomarker discovery and drug development. This review compares recent advancements in homogeneous and heterogeneous protease detection methods, highlighting fluorescence and liquid crystal-based techniques.

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Use of the Protease Fluorescent Detection Kit to Determine Protease Activity

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Published on: August 4, 2009

Area of Science:

Biochemistry
Analytical Chemistry
Biotechnology

Background:

Proteases regulate vital physiological functions and are key biomarkers for diseases like cancer.
The development of protease inhibitors, such as for HIV-1, underscores their therapeutic importance.
Sensitive and selective protease assays are essential for discovering novel proteases and inhibitors.

Purpose of the Study:

To review and compare recent developments in protease activity assays.
To discuss various detection principles for protease assays.
To explore the applications of liquid crystal-based assays for protease detection.

Main Methods:

Comparison of homogeneous and heterogeneous protease assay techniques.
Discussion of fluorescence-based assays for high sensitivity.
Overview of heterogeneous methods including electrochemical, surface spectroscopy, and enzyme-linked peptide assays.
Examination of emerging liquid crystal (LC)-based protease assays.

Main Results:

Homogeneous assays, particularly fluorescence-based, offer high sensitivity but limited multiplexing.
Heterogeneous assays, leveraging microarray technology, enable simultaneous detection of multiple proteases.
Electrochemical, surface spectroscopy, and enzyme-linked peptide assays are common heterogeneous approaches.
Liquid crystal-based assays show promise for protease and inhibitor detection.

Conclusions:

Protease activity assays are vital for advancing diagnostics and therapeutics.
Both homogeneous and heterogeneous assay formats have distinct advantages for protease detection.
Emerging technologies like LC-based assays offer new avenues for sensitive and selective protease analysis.