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Updated: Mar 2, 2026

Estimation of Telomeric Repeat-containing RNA from DNA/RNA Hybrid Complexes
Published on: December 5, 2025
Conformational flexibility in the RNA stem-loop structures formed by CAG repeats
Pei Guo1, Ho Yin Edwin Chan2,3, Sik Lok Lam1
1Department of Chemistry, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong.
Abstract:
The expansion of CAG repeats has been found to be associated with at least nine human genetic disorders. In these disorders, the full-length expanded CAG RNA transcripts are cleaved into small CAG-repeated RNAs which are cytotoxic and known to be capable of forming hairpins. To better understand the RNA pathogenic mechanism, in this study we have performed high-resolution nuclear magnetic resonance structural investigations on the RNA hairpins formed by CAG repeats. Our results show the formation of a type III AGCA tetraloop and reveal the effect of stem rigidity on the loop conformational flexibility.
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