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Translation control: Learning from viruses, again.

Juana Díez1, Jennifer Jungfleisch1

  • 1a Molecular Virology Group, Department of Experimental and Health Sciences , Universitat Pompeu Fabra , Barcelona , Spain.

RNA Biology
|May 11, 2017
PubMed
Summary

Viruses reveal a new RNA translation control mechanism involving DEAD-box helicase Dhh1/DDX6. This process, linked to structured coding sequences and endoplasmic reticulum proteins, is conserved across species.

Keywords:
BMVDhh1RNA secondary structurehelicasetranslational regulationviral RNA

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Area of Science:

  • Molecular Biology
  • Virology
  • Cellular Biology

Background:

  • Viruses are instrumental in elucidating cellular functions.
  • Recent research has focused on understanding viral mechanisms to uncover cellular processes.

Purpose of the Study:

  • To identify and characterize a novel translational control mechanism involving the DEAD-box helicase Dhh1/DDX6.
  • To investigate the role of RNA folding within coding sequences (CDSs) in this mechanism.

Main Methods:

  • Analysis of viral and cellular messenger RNAs (mRNAs).
  • Investigating the binding patterns of Dhh1/DDX6 to mRNAs.
  • Studying the impact of RNA structure on translation initiation.
  • Identifying proteins associated with the endoplasmic reticulum.

Main Results:

  • A novel Dhh1/DDX6-dependent translational control mechanism was identified.
  • Dhh1-dependent mRNAs possess long, structured CDSs and are bound by Dhh1 in a specific pattern.
  • Translation initiation is activated for these mRNAs.
  • Proteins expressed are associated with the endoplasmic reticulum.

Conclusions:

  • A new layer of translation regulation linked to the endoplasmic reticulum has been uncovered.
  • This mechanism is conserved from yeast to humans.
  • Viruses hijack this conserved pathway for their own purposes.