Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Cytokine use in the hematopoietic subsyndrome of acute radiation syndrome (H-ARS): implications for the role of cytokines in a mass casualty radiologic/nuclear (R/N) emergency.

Journal of radiological protection : official journal of the Society for Radiological Protection·2026

Same author

European approach for acute radiation syndrome's management facing radiological and nuclear threats: A call for arms - CORRIGENDUM.

Disaster medicine and public health preparedness·2025

Same author

European Approach for Acute Radiation Syndrome's Management Facing Radiological and Nuclear Threats: A Call for Arms.

Disaster medicine and public health preparedness·2025

Same author

Antiphospholipid IgG Certified Reference Material ERM<sup>®</sup>-DA477/IFCC: a tool for aPL harmonization?

Clinical chemistry and laboratory medicine·2025

Same author

Evaluation of cryoprotein investigation using a digital external quality assurance scheme.

Annals of clinical biochemistry·2024

Same author

Laboratory practice is central to earlier myeloma diagnosis: Utilizing a primary care diagnostic tool and laboratory guidelines integrated into haematology services.

British journal of haematology·2024

Same journal

Routine laboratory triage for NT-proBNP elevation may reflect cardiorenal frailty as much as heart failure specificity.

Annals of clinical biochemistry·2026

Same journal

From interpretable NT-proBNP prediction to equitable laboratory stewardship.

Annals of clinical biochemistry·2026

Same journal

Serum Sclerostin Levels in Children with Osteogenesis Imperfecta.

Annals of clinical biochemistry·2026

Same journal

Population-Specific HOMA-IR Reference Ranges in Iraq: Defining Insulin Sensitivity in a Middle Eastern Cohort.

Annals of clinical biochemistry·2026

Same journal

Optimizing Laboratory Quality with Sigma Metrics: Application of CLIA 2024 Total Allowable Error Guidelines.

Annals of clinical biochemistry·2026

Same journal

Suspected thermal amplitude of cryoglobulin likely more clinically significant than size detected: A case report.

Annals of clinical biochemistry·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Mar 2, 2026

Capillary Electrophoresis Separation of Monoclonal Antibody Isoforms Using a Neutral Capillary

Capillary Electrophoresis Separation of Monoclonal Antibody Isoforms Using a Neutral Capillary

Published on: January 16, 2017

Electrophoretic patterns post daratumumab.

Joanna Sheldon¹, Rachel D Wheeler¹, Ray Powles²

¹1 Protein Reference Unit, 443664 South West London Pathology, St. George's Hospital , London, UK.

Annals of Clinical Biochemistry

|May 12, 2017

Summary

This summary is machine-generated.

Daratumumab (Darzalex), a CD38 monoclonal antibody for multiple myeloma, appears as a small band on serum protein electrophoresis. This finding requires careful interpretation to avoid misdiagnosis of new monoclonal protein development.

Keywords:

Cancer electrophoresis proteins

More Related Videos

Anti-Nuclear Antibody Screening Using HEp-2 Cells

Anti-Nuclear Antibody Screening Using HEp-2 Cells

Published on: June 23, 2014

Simultaneous Distinction of Monospecific and Mixed DFS70 Patterns During ANA Screening with a Novel HEp-2 ELITE/DFS70 Knockout Substrate

Simultaneous Distinction of Monospecific and Mixed DFS70 Patterns During ANA Screening with a Novel HEp-2 ELITE/DFS70 Knockout Substrate

Published on: January 17, 2018

Related Experiment Videos

Last Updated: Mar 2, 2026

Capillary Electrophoresis Separation of Monoclonal Antibody Isoforms Using a Neutral Capillary

Capillary Electrophoresis Separation of Monoclonal Antibody Isoforms Using a Neutral Capillary

Published on: January 16, 2017

Anti-Nuclear Antibody Screening Using HEp-2 Cells

Anti-Nuclear Antibody Screening Using HEp-2 Cells

Published on: June 23, 2014

Simultaneous Distinction of Monospecific and Mixed DFS70 Patterns During ANA Screening with a Novel HEp-2 ELITE/DFS70 Knockout Substrate

Simultaneous Distinction of Monospecific and Mixed DFS70 Patterns During ANA Screening with a Novel HEp-2 ELITE/DFS70 Knockout Substrate

Published on: January 17, 2018

Area of Science:

Clinical Chemistry
Hematology
Immunology

Background:

Daratumumab (Darzalex) is an IgG1 kappa monoclonal antibody targeting CD38.
It is approved for treating refractory multiple myeloma.
As a monoclonal protein, it is detectable via serum protein electrophoresis (SPEP) and immunofixation (SIF).

Purpose of the Study:

To investigate the detectability of daratumumab on routine serum protein electrophoresis.
To inform diagnostic laboratories about potential misinterpretations of daratumumab as a new monoclonal protein.

Main Methods:

Serum samples from four multiple myeloma patients were analyzed.
Electrophoresis was performed immediately before and after daratumumab treatment.

Main Results:

Daratumumab was consistently visible on SPEP as a distinct, small band.
The band appeared in the slow gamma region, approximately 1 g/L.

Conclusions:

Daratumumab is detectable on routine serum protein electrophoresis in multiple myeloma patients.
Diagnostic laboratories must be aware of this finding to prevent misdiagnosis.
Close collaboration between laboratories and clinicians is crucial for accurate interpretation.